Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata  

作　　者：MENG Yao YI Lin CHEN Lei HAO Jie LI Du-Xin XUE Jie XU Nai-Yu ZHANG Zhen-Qing 

机构地区：[1]College of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China [2]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases,Soochow University,Suzhou 215021,China

出　　处：《Chinese Journal of Natural Medicines》2019年第10期792-800,共9页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.81473179 and 81673388);the funding for Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases(No.BM2013003)

摘　　要：Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata(Turcz.) Schischk.(SD) was extracted, fractionated and characterized in this work. Four fractions were prepared. Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR. SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches. SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan. SDP4 was defined as a pectic arabinogalactan. Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1. The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity. The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata(Turcz.) Schischk.(SD) was extracted, fractionated and characterized in this work. Four fractions were prepared. Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR. SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches. SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan. SDP4 was defined as a pectic arabinogalactan. Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1. The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity. The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP.

关 键 词：Saposhnikovia divaricata POLYSACCHARIDES ARABINOGALACTAN ANTIOXIDANT activity 

分 类 号：R284.1[医药卫生—中药学] R965[医药卫生—中医学]