瘦素受体（OB-RGRP）水平对脂肪细胞的影响及其作用机制研究  

The Effect of Leptin Receptor(OB-RGRP) on Adipocytes and Its Mechanism

作　　者：石卫红[1] 李小林[1] 巫国辉[1] 覃煦 万冠群 曾琪 胡云刚 SHI Wei-hong;LI Xiao-lin;WU Guo-hui;QIN Xu;WAN Guan-qun;ZENG Qi;HU Yun-gang(Department of Plastic Maxillofacial Surgery,Jiangxi Provincial People’s Hospital,Nanchang 330006,Jiangxi,China)

机构地区：[1]江西省人民医院整形颌面外科

出　　处：《中国美容医学》2019年第11期90-93,共4页Chinese Journal of Aesthetic Medicine

基　　金：江西省自然科学基金（项目：OB-RGRP调节瘦素受体分布抑制肥胖发病的研究；编号：20122BAB205010）;省科技支撑计划（项目：脂肪细胞自分泌信号传导在肥胖防治中的作用；编号：2010BSA12400）;省科技支撑计划（项目：瘦素-JAK2-STAT3自分泌信号通路在肥胖防治中的作用；编号：20092DS15900）

摘　　要：目的:为了探讨不同水平的瘦素受体(OB-RGRP)调节大鼠脂肪细胞的研究及其作用机制,明确其对脂肪细胞的影响,为瘦素受体治疗肥胖症提供理论依据。方法:原代分离大鼠脂肪细胞,利用油红O染色进行鉴定,构建过表达OB-RGRP和干扰OB-RGRP载体,利用Western blot验证细胞转染效果,并检测JAK2、STAT3及其磷酸化的表达情况。结果:细胞分离正确,构建的OB-RGRP-siRNA转染有效,与对照组相比,过表达OB-RGRP组的p-JAK2和p-STAT3表达明显升高,干扰OB-RGRP组的p-JAK2和p-STAT3表达明显下降,差异具有统计学意义(P<0.05)。而JAK2、STAT3总蛋白各组之间无差异。结论:OB-RGRP水平通过JAK2/STAT3信号通路在调节瘦素抵抗中起着重要作用,调节脂肪代谢,有助于改善瘦素抵抗及肥胖防治。Objective To investigate the mechanism of leptin receptors(OB-RGRP)in regulating obesity In order to investigate the regulation of different levels of leptin receptor on adipocytes in rats and its mechanism of action,and to clarify its effect on adipocytes,so as to provide theoretical basis for the treatment of obesity by leptin receptor.Methods Primary rat adipocytes were isolated and identified by oil red O staining.OB-RGRP overexpressed and OB-RGRP interfering vectors were constructed.The cell transfection effect was verified by Western blot,and the expression of JAK2,STAT3 and their phosphorylation were detected.Results The cells were isolated correctly and the constructed OB-RGRP-siRNA was effective in transfection.Compared with the control group,the overexpression of p-JAK2 and p-STAT3 in the OB-RGRP group was significantly increased,while the expression of p-JAK2 and p-STAT3 in the OB-RGRP group was significantly decreased,and the difference was statistically significant(P<0.05).There was no difference between JAK2 and STAT3 groups.Conclusion OB-RGRP level plays an important role in regulating leptin resistance through the JAK2/STAT3 signaling pathway,regulating fat metabolism and contributing to the improvement of leptin resistance and the prevention and treatment of obesity.

关 键 词：OB-RGRP 瘦素受体 Western BLOT JAK2/STAT3信号通路 脂肪细胞 肥胖症 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]