利用分子标记辅助选育香软型保持系“SH101B”、不育系“SH101A”及配组分析  

Development of selectively aromatic soft maintainer line “SH101B” and sterile line “SH101A” by molecular marker-assisted selection and analysis of hybrid combination

作　　者：王彤 曾文秀 王冬翼 赵国超[1,2] 李建粤[1,2] WANG Tong;ZENG Wenxiu;WANG Dongyi;ZHAO Guochao;LI Jianyue(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Shanghai Engineering Research Center of Plant Germplasm Resources,Shanghai Normal University,Shanghai 200234,China;Agricultural Technology Extension and Service Center of Pudong New Area,Shanghai 201201,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海师范大学上海植物种质资源工程技术研究中心,上海200234 [3]上海市浦东新区农业技术推广中心,上海201201

出　　处：《上海师范大学学报（自然科学版）》2019年第5期550-556,共7页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市农委种业项目(沪农科种字(2016)第1-7号);上海植物种质资源工程技术研究中心项目(17DZ2252700)

摘　　要：为了给三系杂交稻新品种选育提供优良亲本,以“嘉花1号”水稻为转育亲本,与香型软米水稻杂交,再与“嘉花1号”水稻多代回交及自交,结合分子标记辅助选育香软型水稻新品系“SH101B”.“嘉花1号”与“SH101B”水稻主要农艺性状和产量性状差异都不显著(p>0.05).“SH101B”稻米直链淀粉含量(质量分数)为10.0%,明显低于“嘉花1号”水稻(15.2%).又将“SH101B”与三系不育系水稻杂交和回交,转育获得香软型三系不育系“SH101A”,再与籼型恢复系“T201”进行配组,并对后代农艺性状、产量和稻米品质进行分析.结果显示,“SH101A×T201”组合稻米直链淀粉含量为12.8%,每亩产量达到848.1 kg(12726.4 kg·hm^-2).In order to provide excellent parents for breeding new cytoplasmic male sterility(CMS)line rice varieties,we developed a new rice strain,“SH101B”,by molecular marker-assisted gene pyramiding,using hybridizing between non-fragrant and non-soft rice“Jiahua No.1”with a fragrant soft rice,and backcrossing with“Jiahua No.1”rice for multi-generation,and then selfcrossing.There were no significant differences between“Jiahua No.1”and“SH101B”in main agronomic and yield traits(p>0.05).The amylose content(mass fraction)of“SH101B”rice was 10.0%,which was significantly lower than that of“Jiahua No.1”rice(15.2%).In this study,“SH101B”was crossed and backcrossed with three-line sterile line rice to obtain fragrant and soft three-line sterile line“SH101A”,which was then paired with Indica restorer line“T201”,and its offspring were analyzed for agronomic traits,yield and rice quality.The results showed that the amylose content of“SH101A×T201”rice was 12.8%and the yield reached 12726.4 kg·hm^-2.

关 键 词：香软水稻 分子标记辅助选育 “SH101B” “SH101A” 杂交组合 

分 类 号：S511.037[农业科学—作物学]