菠菜乙醇酸氧化酶基因家族的鉴定及表达分析  

Identification and expression analysis of glycolate oxidase gene family in spinach

作　　者：武艺秀 郁征宇 葛晨辉[1,2] 王全华[1,2] 蔡晓锋[1,2] WU Yixiu;YU Zhengyu;GE Chenhui;WANG Quanhua;CAI Xiaofeng(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Shanghai Engineering Research Center of Plant Germplasm Resources,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海师范大学上海植物种质资源工程技术研究中心,上海200234

出　　处：《上海师范大学学报（自然科学版）》2019年第5期557-565,共9页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金青年基金(31501754);上海市科研创新行动计划(16391901000);上海植物种质资源工程技术研究中心项目(17DZ2252700)

摘　　要：在菠菜全基因组中鉴定了菠菜(Spinacia oleracea L.)乙醇酸氧化酶(GLO)家族成员,并对其理化性质、亚细胞定位、基因结构、保守基序、同源关系及基因表达进行了分析,发现菠菜中存在5个SoGLOs蛋白,通过进化树分析,菠菜GLO蛋白与甜菜GLO蛋白亲缘关系较近.通过基因结构分析发现该家族基因由9~11个外显子构成.实时荧光定量聚合酶链式反应(qRT-PCR)结果表明:硝态氮仅能短期诱导SoGLOs的表达,而铵态氮可以持续抑制SoGLOs的表达,从而影响菠菜草酸的含量.在胁迫处理后,SoGLOs的表达均有明显变化,SoGLO1,SoGLO3和SoGLO5对盐胁迫的响应最明显,SoGLOs可能在菠菜的抗盐、耐高温、耐寒、抗旱以及抗氧化过程中起作用.植物激素的喷施普遍使SoGLOs的表达在短时间内增加,这可能引起菠菜体内草酸的快速积累.The spinach(Spinacia oleracea L.)glycolate oxidase(GLO)gene family members were identified from the whole genome of spinach,and their physical and chemical properties,subcellular localization,gene structure,conserved motifs,homology and gene expression were analyzed.Results showed that there are five SoGLOs proteins in spinach.The genetic relationship between spinach’s GLO protein and those of Beta vulgaris L.protein was relatively close through phylogenetic tree analysis.Gene structure analysis revealed that the family gene consisted of 9 to 11 exons.Real-time quantitative polymerase chain reaction(qRT-PCR)showed that nitrate nitrogen could only induce SoGLOs expression in a short period of time,while ammonium nitrogen could continue to inhibit the expression of SoGLOs,thus affecting the content of spinach oxalic acid.After stress treatment,the expression of SoGLOs changed significantly.SoGLO1,SoGLO3 and SoGLO5 responded most to salt stress.SoGLOs may play a role in the salt tolerance,high temperature tolerance,cold tolerance,drought resistance and antioxidant process of spinach.Spraying of phytohormones generally increases the expression of SoGLOs in a short period of time,which may cause rapid accumulation of oxalic acid in spinach.

关 键 词：菠菜(Spinacia OLERACEA L.) 乙醇酸氧化酶(GLO) 逆境胁迫 基因表达 

分 类 号：S636.1[农业科学—蔬菜学]