SAA1在狼疮小鼠肾脏中的表达及其对小鼠肾小管上皮细胞EMT的作用  

Expression of SAA1 in kidney of lupus mice and its effect on EMT of mRTECs

作　　者：周星丞 张帆 严瑞 潘秀超 余洁 潘洪奖 田平平 刘忠强 石明隽[2] 郭兵[2] ZHOU Xing-Cheng;ZHANG Fan;YAN Rui;PAN Xiu-Chao;YU Jie;PAN Hong-Jiang;TIAN Ping-Ping;LIU Zhong-Qiang;SHI Ming-Jun;GUO Bing(Department of Pathophysiology,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州医科大学病理生理学教研室,贵阳550025 [2]贵州医科大学贵州省常见慢性疾病发病机制及药物研究重点实验室,贵阳550025 [3]贵州医科大学附属医院肾内科,贵阳550025

出　　处：《中国免疫学杂志》2019年第21期2561-2565,2575,共6页Chinese Journal of Immunology

基　　金：贵州省科技厅基础研究计划项目(黔科合基础[2019]1272号);贵州省科技厅学术新苗项目(黔科合平台人才[2017]5718-20);贵阳市科技计划项目(筑科合同[2017]30-23号)

摘　　要：目的:探讨SAA1在狼疮小鼠肾脏中的表达及其对肾脏纤维化的影响。方法:选取自发性狼疮肾炎小鼠(LN小鼠)与阴性对照的C57/BL小鼠用于体内实验,应用Masson染色检测其肾脏组织纤维化程度,应用Real-time PCR、Western blot和免疫组化技术检测组织中SAA1以及相关EMT指标的表达情况;体外实验中,将mRTEC细胞应用不同处理后,分为空白对照组、LPS处理组、LPS+Si-NC和LPS+Si-SAA1组,应用Western blot和免疫细胞荧光检测相关蛋白表达。结果:LN小鼠肾脏发生明显纤维化,SAA1表达较正常组明显升高;LPS能诱导mRTECs中SAA1、FN和α-SMA的表达,并降低E-Ca蛋白水平,而同时沉默SAA1能有效减缓LPS这一作用。结论:SAA1在狼疮小鼠中表达明显升高,且能促进肾小管上皮细胞的EMT进程。Objective:To investigate the expression of SAA1 in the kidney of lupus mice and its effect on renal fibrosis.Methods: Spontaneous lupus nephritis mice(LN mice) and C57/BL mice(negative control mice) were selected in vivo experiment.Masson staining was used to detect the degree of renal fibrosis.Real-time PCR,Western blot and immunohistochemical techniques were used to detect the expression of SAA1 and related EMT in tissues.MRTEC cells were divided into blank control group,LPS treatment group,LPS+Si-NC group and LPS+Si-SAA1 group after different treatments.Western blot and immunofluorescence were used to detect the expression of related proteins.Results: LN mice had obvious renal fibrosis,and the expression of SAA1 was significantly higher than that of the normal group.LPS could induce the expression of SAA1,FN and alpha-SMA in mRTECs,and reduce the level of E-Ca protein,while silencing SAA1 could effectively alleviate the effect of LPS.Conclusion: SAA1 expression in lupus mice is significantly increased and can promote the EMT process of renal tubular epithelial cells.

关 键 词：狼疮肾炎 EMT mRTEC细胞 SAA1 

分 类 号：R392[医药卫生—免疫学]