拟南芥AtDGK7基因的组织定位及应答不同胁迫的表达分析  

Tissue localization and expression analyses of AtDGK7 response to different stresses

作　　者：贾羊羊 贾哓玮 司旭阳 张洪艳 张科[1] 潘延云[1] JIA Yangyang;JIA Xiaowei;SI Xuyang;ZHANG Hongyan;ZHANG Ke;PAN Yanyun(Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学河北省植物生理与分子病理学重点实验室

出　　处：《河北农业大学学报》2019年第5期27-32,共6页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金项目(C2017204095);河北省高等学校科学技术研究项目(ZD2017039)

摘　　要：二酰甘油激酶(DGKs)是植物肌醇磷脂信号系统中的关键酶之一,催化二酰甘油(DAG)的磷酸化形成植物中新型的第二信使分子磷脂酸(PA)。拟南芥中AtDGKs有7个亚型。本文利用报告基因GUS和实时定量RT-PCR的方法,检测了拟南芥中AtDGK7亚型基因不同组织的表达特征,以及AtDGK7基因在植物受到各种非生物胁迫及激素刺激下的表达变化。结果显示,AtDGK7在幼苗、根以及花器官中均有表达;该基因在盐和干旱胁迫下表达升高,受到24-eBL的诱导表达,暗示AtDGK7对于拟南芥适应逆境发挥重要作用,同时介导了植物对激素的响应过程。我们的数据为进一步研究AtDGK7的生物学功能提供了基础。Diacylglycerol kinases(DGKs)are one of the key enzymes in the plant inositol phospholipid signaling system,which catalyzes the phosphorylation of diacylglycerols(DAGs)to form the novel second messenger molecule phosphatidic acid(PA)in plants.There are 7 subtypes of AtDGKs in Arabidopsis.In this paper,the expression characteristics of AtDGK7 subtype gene in Arabidopsis thaliana were detected by reporter gene GUS and real-time quantitative RT-PCR.The transcripts of AtDGK7 in plants was increasing under various abiotic stresses and hormone treatments.Our results showed that AtDGK7,which were expressed in seedlings,roots and floral organs,was significantly stimulated in transcriptional level by salt and drought,and induced by 24-eBL stimulation.The data from the expression analysis provided the basis for further study of the function of AtDGK7.

关 键 词：拟南芥 AtDGK7 组织定位 基因表达 胁迫刺激 

分 类 号：Q71[生物学—分子生物学]