生长素IAA对荷花花期调控的影响  

The Influence of Auxin IAA on the Flowering Regulation of Lotus

作　　者：刘艺平[1] 黄志远 梁露 孟亚南[1] 孔德政[1] LIU Yiping;HUANG Zhiyuan;LIANG Lu;MENG Yanan;KONG Dezheng(College of Forestry,Agricultural University of Henan,Zhengzhou 450002,China)

机构地区：[1]河南农业大学林学院

出　　处：《河南农业科学》2019年第11期141-145,共5页Journal of Henan Agricultural Sciences

基　　金：河南省重大科技专项(30400098)

摘　　要：为探讨盆栽荷花在不同质量浓度外源IAA处理下的花期变化,以荷花品种青玉为试验材料,选择长势相同的荷花进行IAA针管注射及创伤涂抹处理,IAA质量浓度设置为0(对照,采用同体积的蒸馏水进行注射)、100、150、200 mg/L,分析各处理的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量变化,并分析其抗性生理指标间的相关性。结果表明,外源IAA胁迫均使荷花花瓣的长宽及最大花径得到了不同程度的增加,观赏效果更好。150 mg/L IAA处理使初花期提前2 d,延长了荷花的单花寿命。150 mg/L IAA处理的SOD、POD、CAT活性最大值均显著高于对照组10.15%、58.70%、13.40%。随着处理时间的增加,MDA含量持续上升,其中150 mg/L IAA处理下其积累量在各个时期均较对照组低,而其余处理变化不明显。谢花期的SOD活性分别与POD活性和CAT活性呈现极显著正相关,POD、CAT活性呈现显著正相关,三者又分别与MDA含量呈现极显著负相关,说明当荷花受到外源胁迫时,体内各种保护酶之间是相互作用的,防止膜脂过氧化,减缓植物细胞衰老,使花期延长。综上,150 mg/L IAA处理使荷花拥有较好的观花品质并显著提高了各种保护酶的生理活性,减缓了活性氧的积累,延长了荷花的观赏期。In order to investigate the anthesis changes of potted lotus under different mass concentrations of exogenous IAA,the lotus variety sapphire was used as the experimental material,and the lotus flowers with the same growth were selected for auxin injection and wound application.The IAA mass concentration was set as follows:0(CK,injected with distilled water of the same volume),100,150 and 200 mg/L were used to analyze the changes in SOD,POD,CAT activities and malondialdehyde(MDA)content of each treatment,and to analyze the correlation between the physiological indicators of resistance.The results showed that exogenous auxin stress increased the length,width and maximum diameter of lotus petals to different degrees,and the ornamental effect was better.The treatment with 150 mg/L IAA advanced the initial flowering period by 2 d,and extended the single flower life of lotus.The maximum values of SOD,POD and CAT activities in 150 mg/L IAA treatment were significantly higher than those in the control group by 10.15%,58.70%,13.40%.With the increase of treatment time,the MDA content continued to rise,and the accumulation of 150 mg/L IAA treatment was lower than that of the control at each period,while changes of the other treatments were not obvious.At flower-wilting stage,SOD activity and POD,CAT activities showed very significantly positive correlation,POD activity and CAT activity showed significantly positive correlation,and the three showed a significantly negative correlation with MDA content,respectively,indicated that when lotus suffered exogenous stress,a variety of protective enzymes interacted,prevented membrane lipid peroxidation,slowed down aging of plant cells,and extended flowering period.In summary,150 mg/L IAA treatment made lotus flower have better flower quality and significantly improved the physiological activity of various protective enzymes,slowed down the accumulation of reactive oxygen species and extended the ornamental period of lotus flower.

关 键 词：生长素 荷花 花期延长 生理指标 吲哚-3-乙酸 

分 类 号：S682.32[农业科学—观赏园艺]