辣椒白粉病抗性QTL定位分析  

QTL Mapping Analysis of Powdery Mildew Resistance in Pepper

作　　者：白锐琴 张正海[1] 曹亚从 于海龙[1] 朱砚姝 刘婧[1] 张南南 王立浩[1] 张宝玺[1] BAI Rui-qin;ZHANG Zheng-hai;CAO Ya-cong;YU Hai-long;ZHU Yan-shu;LIU Jing;ZHANG Nan-nan;WANG Li-hao;ZHANG Bao-xi(Institute of Vegetables&Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所

出　　处：《中国蔬菜》2019年第11期23-29,共7页China Vegetables

基　　金：国家重点研发计划项目(2017YFD0101900);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业农村部园艺作物生物学与种质创制重点实验室项目

摘　　要：以辣椒(Capsicum annuum)白粉病抗、感病亲本H3和83-60构建的包含142个重组自交系的RIL(H3×83-60)F8群体为试验材料,基于亲本重测序数据设计KASPar引物,构建了包含16个连锁群的辣椒遗传连锁图谱,总图距1 356.5cM,标记间平均图距为4.69 cM。基于2016年和2017年重组自交系群体白粉病发病表型数据,获得了PMR6.1、PMR9.1、PMR11.1、PMR11.2和PMR5.1等5个白粉病抗性QTL位点,其中PMR6.1、PMR9.1在两年中均被检测到,表型贡献率在10.8%~21.4%之间。通过辣椒白粉病抗性QTL定位,开发与白粉病抗性基因连锁的SNP分子标记,对于辣椒抗病辅助育种有重要参考价值。Taking 142 recombinant inbred lines(‘H3’ב83-60’)F8 group developed by crossing powdery mildew resistant parental line‘ H3’(Capsicum annuum) and susceptive parental line‘ 83-60’(C.annuum)as studying material,and based on KASPar primer developed by re-sequencing data of parental lines,this paper constructed pepper genetic linkage map including 16 linkage groups.The total map distance was 1 356.5 cM,with an average distance of 4.69 cM between adjacent markers.Based on the phenotypic data of recombinant inbred lines in 2016 and 2017,5 QTLs naming PMR6.1,PMR9.1,PMR11.1,PMR11.2 and PMR5.1 resistant to powdery mildew were detected.Among them,PMR6.1 and PMR9.1 were detected in these 2 years.The phenotypic contribution rate was between 10.8%-21.4%.This study developed the SNP molecular marker chain-operated with powdery mildew resistance gene was of important reference value for pepper disease resistance assistant breeding.

关 键 词：辣椒 白粉病 遗传图谱 QTL定位 

分 类 号：S43[农业科学—农业昆虫与害虫防治]