应激相关microRNA在糖尿病足并发感染患者中的表达研究  

Expression and significance of stress-related microRNA in diabetic foot patients complicated with infection

作　　者：陈书程 王爱芳[1] 邵晓云[2] 毛齐彬[1] 杨磊[1] CHEN Shu-cheng;WANG Ai-fang;SHAO Xiao-yun;MAO Qi-bin;YANG Lei(Shaoxing Second Hospital,Shaoxing,Zhejiang312000,China)

机构地区：[1]绍兴第二医院内分泌科,浙江绍兴312000 [2]绍兴第二医院重症监护室,浙江绍兴312000

出　　处：《中华医院感染学杂志》2019年第20期3111-3115,3138,共6页Chinese Journal of Nosocomiology

基　　金：浙江省康恩贝医院管理软科学研究基金资助项目(2018ZHA-KEB331)

摘　　要：目的探究应激相关微小RNA(microRNA)在糖尿病足合并感染患者中的表达。方法选择2017年3月-2018年7月于绍兴第二医院接受治疗的糖尿病足合并感染患者78例,并设为试验组,匹配同期于医院治疗的糖尿病合并糖尿病足未发生感染患者78例为对照组。采集患者临床资料,检测患者总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、空腹血糖(FPG)及糖化血红蛋白(HbA1c)等水平。采用生物信息学方法对相关应激microRNA进行筛选,并对应激相关microRNA表达进行检测。结果试验组患者TC、TG、LDL-C、FPG及HbA1c分别为(5.67±1.21)mmol/L、(3.01±0.72)mmol/L、(3.09±0.76)mmol/L、(9.39±2.70)mmol/L及(7.21±1.32)%高于对照组(P<0.05);试验组患者促肾上腺皮质激素释放激素、IL-6、血浆肾上腺素、去甲肾上腺素及皮质醇分别为(5.67±0.51)μg/L、(134.27±14.23)ng/L、(157.82±43.29)ng/L、(423.18±93.27)ng/L及(223.29±36.37)μg/L高于对照组患者(P<0.05);所有研究对象促肾上腺皮质激素释放激素与microRNA-142水平呈负相关,而IL-6、肾上腺素与去甲肾上腺素与Let-7b呈正相关性,且与microRNA-142水平呈负相关(P<0.05)。皮质醇的水平与microRNA-144、miRNA-29a及Let-7b呈正相关性,且与microRNA-142呈负相关(P<0.05);试验组促肾上腺皮质激素释放激素、血浆肾上腺素、去甲肾上腺素与Let-7b水平呈正相关,皮质醇与microRNA-144及microRNA-29a的水平也呈现正相关性,但IL-6水平与microRNA-142呈负相关(P<0.05)。结论试验组糖尿病足患者microRNA-144和Let-7b增加,而microRNA-142则有所下降;microRNA-144、Let-7b及microRNA-142可能是慢性应激状态下糖尿病足患者发生发展的相关调节因子,这对于应激相关microRNA在糖尿病足患者的诊治和预防上提供一定的方向,值得探究。OBJECTIVE To investigate the expression of stress-related microRNA(microRNA)in patients with diabetic foot complicated with infection.METHODS 78patients with diabetic foot complicated with infection who were treated in hospital from Mar.2017to Jul.2018were selected as the experimental group.At the same time,78patients with diabetes mellitus complicated with diabetic foot infection in the same period were selected as the control group.The clinical data were collected and the levels of total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein chotesterol(LDL-C),fasting blood glucose(FPG)and glycated hemoglobin A1c(HbA1c)were measured.The stress-related microRNA was screened by bioinformatics method,and the expression level of stress-related microRNA was detected by real-time fluorescence quantitative PCR.RESULTS TC,TG,LDL-C,FPG and HbA1cin the experimental group were(5.67±1.21)mmol/L,(3.01±0.72)mmol/L,(3.09±0.76)mmol/L,(9.39±2.70)mmol/L and(7.21±1.32)%,higher than those in the control group(P<0.05).Corticotropin-releasing hormone,interleukin-6(IL-6),plasma adrenaline,norepinephrine and cortisol in the experimental group were(5.67±0.51)μg,(134.27±14.23)ng/L,(157.82±43.29)ng/L,(423.18±93.27)ng/L and(223.29±36.37)μg/L,respectively,higher than those of the control group(P<0.05).The corticotropin-releasing hormone was negatively correlated with microRNA-142level,while IL-6,adrenaline and norepinephrine were positively correlated with Let-7b,and negatively correlated with microRNA-142level(P<0.05).The levels of cortisol were positively correlated with microRNA-144,miR29aand Let-7b,and negatively correlated with microRNA-142(P<0.05).In the experimental group,corticotropin-releasing hormone,plasma adrenaline and norepinephrine were positively correlated with the level of Let-7b.Cortisol was also positively correlated with microRNA-144and microRNA-29alevels,but IL-6level was negatively correlated with microRNA-142(P<0.05).CONCLUSIONCompared with the control group,

关 键 词：应激 糖尿病足 感染 微小核糖核酸 规律 

分 类 号：R587.1[医药卫生—内分泌]