五味子乙素对大鼠心室肌细胞钠电流的影响  

Effects of schisandrin B on sodium channel currents of rats’ ventricular myocardial cells

作　　者：邹丽 王秀秀 钱薇 杨蕴智 葛焱 许正新[1,2,3,4] ZOU Li;WANG Xiu xiu;QIAN Wei;YANG Yun zhi;GE Yan;XU Zheng xin(College of Medicine,Yangzhou University,Yangzhou,225000,China;Jiangsu Key Laboratory for Prevention and Control of Animal Infectious Diseases and Zoonosis,Yangzhou 225001,China;Jiangsu Provincial Key Laboratory of Integrative Medicine for Prevention and Treatment of Diseases in Elderly,Yangzhou 225001,China;Jiangsu Key Laboratory of Basic Medical Sciences and Clinical Transformation for Non coding RNAs,Yangzhou 225009,China)

机构地区：[1]扬州大学医学院,江苏扬州225000 [2]江苏省重点动物传染病和人畜共患病预防控制共创中心,江苏扬州225001 [3]江苏省中西医结合老年病防治重点实验室,江苏扬州225001 [4]江苏省非编码RNA基础与临床转化重点实验室,江苏扬州225009

出　　处：《中成药》2019年第11期2608-2613,共6页Chinese Traditional Patent Medicine

基　　金：扬州大学大学生科研创新计划项目(x20180729)

摘　　要：目的探讨五味子乙素对大鼠心室肌细胞钠电流(INa)的影响。方法应用Langendorff主动脉逆向灌流、单酶解法获得单个大鼠心室肌细胞,采用全细胞膜片钳技术记录不同浓度的五味子乙素对大鼠心室肌细胞膜上INa的影响。结果五味子乙素(>3μmol/L)对INa可产生显著抑制作用(P<0.05,P<0.01),并呈浓度依赖性。五味子乙素(10、30μmol/L)可使钠通道的I-V曲线显著上移,但I-V曲线的激活电位、峰电位及形态未发生改变,使INa的激活曲线向去极化方向迁移(P<0.01),半数激活电位由给药前(-53.90±5.21) mV变为(-43.69±5.34) mV和(-40.80±3.23) mV。五味子乙素能使INa失活曲线向超级化方向移动(P<0.01),半数失活电压(对照、10μmol/L、30μmol/L)分别为(-57.80±6.21) mV、(-71.16±6.45) mV和(-81.03±7.53) mV。此外,五味子乙素还能显著延长INa失活后恢复时间(P<0.01),τ(对照、10μmol/L、30μmol/L)分别为(16.68±1.72) ms、(25.73±2.93) ms和(43.79±3.87) ms。结论五味子乙素能浓度依赖性阻滞大鼠心室肌细胞INa,对其激活、失活和失活后恢复动力学特征均有影响。AIM To observe the effects of schisandrin B(Sch B) on sodium current(INa) in rats ventricular myocytes. METHODS Single rats ventricular myocytes were obtained by reverse perfusion of Langendorff aorta and single enzymatic hydrolysis. Whole cell patch clamp technique was used to record the effect of different concentrations of Sch B on INa of rats ventricular myocytes.RESULTS Sch B significantly inhibited INa in a concentration-dependent manner. Sch B at concentrations of 10 μmol/L and 30 μmol/L raised the IV curve of the sodium current, brought no change to the activation potential, peak potential and morphology of the IV curve, and shifted the activation curve of INa to the depolarization direction, with the half activation potential varying from(-53.90±5.21) mV, a level found prior to the intervention to levels of(-43.69±5.34) mV and(-40.80±3.23) mV(P<0.01) after the intervention. Additionally, Sch B at concentrations of 10 μmol/L and 30 μmol/L shifted the INa inactivation curve to the super-direction(P<0.01), as half inactivation voltages variations were observed in the two groups(-71.16±6.45) mV and(-81.03±7.53) mV, in contrast to the control group’s(-57.80±6.21) mV. Futhermore, Sch B significantly prolonged the recovery time after INa inactivation(P<0.01), as evidenced by τ increaing from(16.68±1.72) ms in control group to(25.73±2.93) ms and(43.79±3.87) ms in the intervention groups.CONCLUSION Sch B can block the INa of rats ventricular myocytes in a concentration-dependent manner, and it has an effect on the activation kinetics after activation, inactivation and post inactivation recovery.

关 键 词：五味子乙素 心室肌细胞 膜片钳 钠通道 

分 类 号：R285[医药卫生—中药学]