HPLC法测定不同饲草料中8种维生素的含量  

Determination of eight kinds of vitamins in different forages by HPLC

作　　者：王敏 李晓龙[3] 许莲华 王永贵 云水丽 韩天龙 李小兵 刘国文[1] WANG Min;LI Xiao-long;XU Lian-hua

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]赤峰市农牧科学研究院,内蒙古赤峰024031 [3]内蒙古林西县农牧业局,内蒙古赤峰025250

出　　处：《饲料研究》2019年第10期62-65,共4页Feed Research

基　　金：内蒙古自治区科技计划项目“肉牛日粮和牛肉营养特征模型的构建应用（项目编号：201601036）”;内蒙古农牧业科学院青年创新基金项目（项目编号：2018QNJJM09)

摘　　要：试验采用高效液相色谱法,对蒙东地区常见的6种饲草料,进行了8种维生素含量的检测分析。结果显示,新鲜牧草中VE、VA和VB2的含量最高,分别为65.99、0.35、373.21 mg/kg;玉米秸秆中VC含量最高,为454.16 mg/kg;谷草中VB12含量最高,为12.84 mg/kg;仅谷草中含有VB1,含量为94.06 mg/kg;仅青干草中含有VB6,含量为72.75 mg/kg;6种饲草料样本中均未检出VD。研究为畜牧生产中饲草料的配制和检测提供参考。HPLC was used to detect eight kinds of vitamins levels in six different forages in eastern Inner Mongolia. The results showed that the contents of VE, VA and VB2 were the highest in fresh herbage, with 65.99, 0.35 and 373.21 mg/kg, respectively. The content of VC was the highest in corn straw, which was 454.16 mg/kg. The content of VB12 was the highest in millet straw, which was 12.84 mg/kg. Only millet straw contented VB1, which was 94.06 mg/kg. Only green hay contented VB6, which was 72.75 mg/kg. VD was not found in six kinds of forages. The study would provide a reference for the preparation and detection of forage in livestock production.

关 键 词：饲草料 维生素 高效液相色谱 

分 类 号：S816.17[农业科学—饲料科学]