健脾解毒活血汤对幽门螺杆菌相关性胃炎脾胃湿热证模型小鼠IL-1β、GAS的影响  

Effect of Jianpi Jiedu Huoxue decoction on interleukin-1β and gastrin in mice with Helicobacter pylori-associated gastritis with spleen-stomach damp-heat syndrome

作　　者：胡锦洋[1] 蒋士生[2] 肖梅英[3] 徐琳本[3] 李勇敏[1] HU Jinyang;JIANG Shisheng;XIAO Meiying;XU Linben;LI Yongmin(The Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China;Hunan Academy of ChiHese Medicine,Changsha 410006,Hunan,China;Institute of Chinese Materia Medica,Hunan Academy of Chinese Medicine,Changsha 410013,Hunan,China)

机构地区：[1]湖南省中医药研究院附属医院,湖南长沙410006 [2]湖南省中医药研究院,湖南长沙410006 [3]湖南省中医药研究院中药研究所,湖南长沙410013

出　　处：《湖南中医杂志》2019年第10期144-146,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省教育厅资助项目(编号:12A104)

摘　　要：目的:观察健脾解毒活血汤对幽门螺旋杆菌相关性胃炎(HAG)脾胃湿热证模型小鼠血清白介素-1β(IL-1β)、胃泌素(GAS)及胃内组织Hp感染数的影响,为研制新型抗Hp感染的中药提供实验依据。方法:选取40只SPF级昆明小鼠为实验对象,将其随机分为空白组、模型组、西药组和中药组,每组各10只。采用Hp菌液灌胃以建立HAG脾胃湿热证小鼠模型,西药组予以奥美拉唑钠肠溶胶囊灌胃,中药组予以健脾解毒活血汤灌胃,均连续给药14d。观察比较各组小鼠血清IL-1β、GAS与胃内组织Hp感染情况。结果:模型组小鼠血清IL-1β、GAS的含量较空白组明显升高(P<0.01,P<0.05);与模型组比较,中药组和西药组血清IL-1β显著降低(P<0.05,P<0.01),GAS水平2组无明显差异。模型组小鼠胃组织Hp感染数较空白组明显增加(P<0.01),中药组和西药组胃组织Hp感染数较模型组显著降低(P<0.05)。结论:健脾解毒活血汤对HAG脾胃湿热证模型小鼠血清IL-1β及Hp感染数有明显改善作用,但对GAS影响不大,在一定程度上可有效治疗脾胃湿热型HAG。Objective:To investigate the effect of Jianpi Jiedu Huoxue decoction on the serum levels of interleukin-1β(IL-1β)and gastrin(GAS)and the number of cells with Helicobacter pylori(Hp)infection in gastric tissue in mice with Hp-associated gastritis(HAG)with spleen-stomach damp-heat syndrome,and to provide an experimental basis for the development of new traditional Chinese medicine(TCM)drugs for Hp infection.Methods:A total of 40 specific pathogen-free Kunming mice were selected and randomly divided into blank group,model group,Western medicine group,and TCM group,with 10 mice in each group.A mouse model of HAG with spleen-stomach damp-heat syndrome was established by Hp bacterial solution by gavage.The mice in the Western medicine group were given omeprazole sodium enteric-coated capsules by gavage,and those in the TCM group were given Jianpi Jiedu Huoxue decoction by gavage;both groups were treated for 14 days.The serum levels of IL-1β and GAS and Hp infection in gastric tissue were compared between groups.Results:The model group had significantly higher serum levels of IL-1β and GAS than the blank group(P<0.01,P<0.05);compared with the model group,the TCM group and the Western medicine group had a significant reduction in the serum level of IL-1β(P<0.05,P<0.01),while there was no significant difference in the level of GAS.Compared with the blank group,the model group had a significant increase in the number of cells with Hp infection in gastric tissue(P<0.01),and compared with the model group,the TCM group and the Western medicine group had a significant reduction in the number of cells with Hp infection in gastric tissue(P<0.05).Conclusion:Jianpi Jiedu Huoxue decoction can significantly improve serum IL-1β level and number of cells with Hp infection in mice with HAG with spleen-stomach damp-heat syndrome,with little effect on GAS,and thus exerts a certain therapeutic effect on HAG with spleen-stomach damp-heat syndrome.

关 键 词：胃炎 幽门螺杆菌 脾胃湿热证 健脾解毒活血汤 IL-1Β GAS 实验研究 

分 类 号：R259.733[医药卫生—中西医结合]