云贵川湖泊芽孢杆菌分离、鉴定及抗动物病原菌活性研究  

Isolation and Identification of Bacillus with Antimicrobial Activity from the Lakes of Yunnan, Guizhou, and Sichuan Provinces

作　　者：张君利 刘超兰 郭义东 林家富 ZHANG Junli;LIU Chaolan;GUO Yidong;LIN Jiafu(Sichuan Industrial Institute of Antibiotics,Chengdu University,Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province,Chengdu 610052,China)

机构地区：[1]成都大学四川抗菌素工业研究所抗生素研究与再评价四川省重点实验室

出　　处：《四川动物》2019年第6期686-694,共9页Sichuan Journal of Zoology

基　　金：中国湖泊微生物多样性及资源调查项目(2017FY100301);四川省医药微生物共享服务平台建设项目(2019JDPT0012);四川省教育厅科研项目(18ZB0137);四川省科技计划项目(2019YJ0282);四川省环境保护科技计划项目(2018HB27)

摘　　要：为从湖泊沉积物中筛选出对动物病原菌具有抑菌活性的芽孢杆菌Bacillus,采用纯培养方法对云贵川地区9个湖泊样品中的芽孢杆菌进行分离、16S rRNA基因序列测定和发酵,以4株常见动物病原菌作为对象,对芽孢杆菌次级代谢产物进行活性检测,筛选有抗菌活性的菌株,并对活性菌株的发酵产物进行液相色谱-质谱联用技术分析。结果显示,在9个湖泊样品中共分离出47株芽孢杆菌,筛选出13株有抗菌活性的菌株,且其产生的脂肽类抗生素对动物病原菌显示出较好的抗菌活性。本研究不仅扩大了芽孢杆菌物种资源信息库,为后续开展芽孢杆菌次生代谢产物研究奠定理论依据。同时也为开发新型动物致病菌抗生素提供了新的途径。To test the antimicrobial activities of Bacillus species, a total of 244 bacterial strains were previously isolated from the sediments of lakes in Yunnan, Guizhou, and Sichuan provinces of China. The strains were identified by sequencing the 16S rRNA gene. After fermentation, the antimicrobial activities of the secondary metabolites of Bacillus was determined by using 4 strains of common animal pathogenic bacteria, Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , and Candida albicans . The fermented products of strains were analyzed by High Performance Liquid Chromatography-Mass Spectrometry. The results showed that 47 isolates from 9 lake samples were identified as Bacillus , and 13 of which were found to have antimicrobial activities by producing the lipopeptide antibiotics. This study expands the current understanding of Bacillus species resources, lays a theoretical foundation for further studies on the secondary metabolites of Bacillus , and also opens a new avenue for the discovery of antibiotics against animal pathogens.

关 键 词：湖泊沉积物 芽孢杆菌 动物病原菌 抗菌活性 

分 类 号：R978.1[医药卫生—药品]