Simultaneous Determination of Four Ingredients in Guanxin Danshen Tablets by RP-HPLC  

作　　者：Yougen WU Guihua ZENG Daoan XIAO Yujun WU Yanjun CHEN Juan LIU 

机构地区：[1]Nanchang Vocational University,Nanchang 330050,China [2]Nanchang County Hospital of Traditional Chinese Medicine,Nanchang 330202,China [3]Institute of Chemistry and Biological Engineering,Yichun University,Yichun 336000,China [4]Jiangxi Normal University,Nanchang 330004,China

出　　处：《Medicinal Plant》2019年第5期18-19,27,共3页药用植物：英文版

基　　金：Supported by Science and Technology Research Project of Jiangxi Provincial Department of Education(GJJ181381)

摘　　要：[Objectives]This study aimed to establish a method for simultaneous determination of four ingredients in Guanxin Danshen tablets by RP-HPLC.[Methods]HPLC chromatography was adopted with column of Thermo Hypersil-Keystone C 18 column(4.6 mm×250 mm,5μm),mobile phase of acetonitrile(A)-0.03 mol/L ammonium acetate(pH adjusted to 2.4 with formic acid)(B),gradient elution(0-5 min,5%A;5-10 min,5%A→19%A;10-40 min,19%A;40-68 min,19%A→36%A;68-90 min,36%A→95%A),flow rate of 1.0 mL/min and column temperature of 25℃.[Results]The content of salvianic acid A sodium,protocatechuic aldehyde,salvianolic acid B and tanshinone II A showed good linear relationship with chromatographic peak area in the range of 3.310-18.66,0.03950-0.2370,0.7500-4.500,0.05920-0.3550μg,respectively.The recovery rate(n=6)was 101.75%,96.86%,104.15%and 99.03%,respectively,and the RSD was 1.52%,2.81%,1.80%,and 1.37%respectively.The established method has good precision,reproducibility and stability.[Conclusions]This method can be used for the quality control of multiple ingredients of Guanxin Danshen tablets.[Objectives] This study aimed to establish a method for simultaneous determination of four ingredients in Guanxin Danshen tablets by RP-HPLC. [Methods] HPLC chromatography was adopted with column of Thermo Hypersil-Keystone C18 column（4.6 mm × 250 mm, 5 μm）, mobile phase of acetonitrile（A）-0.03 mol/L ammonium acetate（pH adjusted to 2.4 with formic acid）（B）, gradient elution（0-5 min, 5% A; 5-10 min, 5% A→19% A; 10-40 min, 19% A; 40-68 min, 19% A→36% A; 68-90 min, 36% A→95% A）, flow rate of 1.0 mL/min and column temperature of 25 ℃. [Results] The content of salvianic acid A sodium, protocatechuic aldehyde, salvianolic acid B and tanshinone IIA showed good linear relationship with chromatographic peak area in the range of 3.310-18.66, 0.039 50-0.237 0, 0.750 0-4.500, 0.059 20-0.355 0 μg, respectively. The recovery rate（n=6） was 101.75%, 96.86%, 104.15% and 99.03%, respectively, and the RSD was 1.52%, 2.81%, 1.80%, and 1.37% respectively. The established method has good precision, reproducibility and stability. [Conclusions] This method can be used for the quality control of multiple ingredients of Guanxin Danshen tablets.

关 键 词：HPLC SIMULTANEOUS determination Guanxin DANSHEN TABLETS 

分 类 号：R28[医药卫生—中药学]