Simultaneous Determination of Four Amino Acids in Fufang Ejiao Buxue Granule by HPLC  

作　　者：Ningning YIN Dongxiao GUO 

机构地区：[1]Shandong Center for Food and Drug Evaluation&Certification,Jinan 250014,China [2]Shandong Institute for Food and Drug Control,Jinan 250101,China

出　　处：《Medicinal Plant》2019年第5期20-22,49,共4页药用植物：英文版

摘　　要：[Objectives]To establish an High Performance Liquid Chromatography(HPLC)method for simultaneous determination of four amino acids in Fufang Ejiao Buxue Granule.[Methods]The quantitative analysis was carried out with a Waters Sunfire C 18 column(4.6 mm×250 mm,5μm).A binary mobile solvent was used:mobile solvent A was acetonitrile-0.1 mol/L sodium acetate solution(adjusting pH to 6.5 with 36%acetic acid)(7∶93)and mobile solvent B was acetonitrile-H 2O(4∶1).The mobile phase was delivered at a flow rate of 1.0 mL/min with a gradient elution profile(0-13 min,100%A→93%A;13-17.9 min,93%A→88%A;17.9-29.0 min,88%A→85%A;29-39 min,85%A→66%A;39-45 min,66%A→0%A).The column temperature was at 43℃.The detection wavelength was 254 nm.[Results]The injection volume of L-hydroxyproline,glycine,alanine,and L-proline showed a good linear relationship with the chromatographic peak area in the range of 0.012 to 0.117,0.022 to 0.218,0.010 to 0.097,0.016 to 0.160μg,separately.The average recovery rate(n=6)was 96.4%,97.3%,97.1%,and 99.4%,respectively;the relative standard deviations were 1.2%,1.9%,1.7%,and 0.9%,respectively.[Conclusions]This method is simple in operation and good in reproducibility,and provides a reliable method for controlling the quality of Fufang Ejiao Buxue Granules.[Objectives] To establish an High Performance Liquid Chromatography（HPLC） method for simultaneous determination of four amino acids in Fufang Ejiao Buxue Granule. [Methods] The quantitative analysis was carried out with a Waters Sunfire C18 column（4.6 mm × 250 mm, 5 μm）. A binary mobile solvent was used: mobile solvent A was acetonitrile-0.1 mol/L sodium acetate solution（adjusting pH to 6.5 with 36% acetic acid）（7∶93） and mobile solvent B was acetonitrile-H2O（4∶1）. The mobile phase was delivered at a flow rate of 1.0 mL/min with a gradient elution profile（0-13 min, 100% A → 93% A; 13-17.9 min, 93% A → 88% A; 17.9-29.0 min, 88% A → 85% A; 29-39 min, 85% A → 66% A; 39-45 min, 66% A → 0% A）. The column temperature was at 43 ℃. The detection wavelength was 254 nm. [Results] The injection volume of L-hydroxyproline, glycine, alanine, and L-proline showed a good linear relationship with the chromatographic peak area in the range of 0.012 to 0.117, 0.022 to 0.218, 0.010 to 0.097, 0.016 to 0.160 μg, separately. The average recovery rate（n=6） was 96.4%, 97.3%, 97.1%, and 99.4%, respectively; the relative standard deviations were 1.2%, 1.9%, 1.7%, and 0.9%, respectively. [Conclusions] This method is simple in operation and good in reproducibility, and provides a reliable method for controlling the quality of Fufang Ejiao Buxue Granules.

关 键 词：Fufang Ejiao Buxue GRANULE Amino acid L-hydroxyproline GLYCINE ALANINE L-PROLINE 

分 类 号：O62[理学—有机化学]