硒对线粒体STAT3活性表达及细胞凋亡的影响研究  

Study on effects of selenium on mitochondrial STAT3 activity expression and cellular apoptosis

作　　者：杨胜祥 张旭涛 华晓芳 YANG Shengxiang;ZHANG Xutao;HUA Xiaofang(Central Hospital of Enshi Tujia and Miao Autonomous Prefecture/Enshi Clinical College,Wuhan University,Enshi,Hubei 445000,China;Department of Cardiology,Jianshi County Hospital of Traditional Chinese Medicine,Enshi,Hubei 445300,China)

机构地区：[1]湖北省恩施土家族苗族自治州中心医院/武汉大学恩施临床学院,湖北恩施445000 [2]湖北省建始县中医医院心内科,湖北恩施445300

出　　处：《重庆医学》2019年第21期3618-3621,共4页Chongqing medicine

基　　金：2017-2018年度湖北省卫生健康委适宜技术推广项目(WJ2017S019)

摘　　要：目的研究硒对心肌细胞线粒体信号转导和转录激活因子3(STAT3)与琥珀酸脱氢酶活性及细胞凋亡的影响。方法原代培养乳鼠心肌细胞,根据不同硒浓度培养液分为无毒性对照组(0.1μmol/L亚硒酸钠)及低硒组(0.05μmol/L亚硒酸钠)。免疫印迹检测线粒体STAT3、p-STAT3及凋亡相关蛋白Bax、Bcl-2的表达,分光光度计测定琥珀酸脱氢酶活性,流式细胞术检测心肌细胞凋亡情况。结果与对照组相比,低硒组琥珀酸脱氢酶活性明显降低,差异有统计学意义(P<0.05)。低硒组线粒体STAT3活性与对照组相比明显降低(P<0.01),但总STAT3差异无统计学意义(P>0.05)。与对照组相比,低硒组心肌细胞Bax蛋白表达升高,Bcl-2蛋白表达降低,细胞凋亡率增加,差异有统计学意义(P<0.05)。结论硒缺乏导致心肌细胞线粒体STAT3活性及线粒体功能下降,促进心肌细胞凋亡。Objective To study the influence of selenium on mitochondrial signal transduction and activator of transcription 3(STAT3),the activity of succinate dehydrogenase and cellular apoptosis.Methods The neonatal rat cardiomyocytes performed the primary culture,and were divided into the non-toxic control group(0.1μmol/L sodium selenite)and low selenium treatment group(0.05μmol/L sodium selenite)according to different selenium concentrations of culture solution.The expressions of mitochondria STAT3,p-STAT3 and apoptosis related proteins,Bax and Bcl-2,were detected by the immunoblotting.The activity of succinate dehydrogenase and the apoptosis of cardiomyocytes were detected by using the spectrophotometer and flow cytometry respectively.Results Compared with the control group,the activity of succinate dehydrogenase in the low selenium treatment group was significantly decreased,and the difference was statistically significant(P<0.05).The activity of STAT3 in the low selenium treatment group was significantly decreased compared with the control group(P<0.01),but no statistical significance difference was found in the total STAT3(P>0.05).Compared with the control group,the myocardial cellular Bax protein expression level and the cellular apoptosis in the low selenium treatment group were increased,while the Bcl-2 protein expression level was decreased,and the differences were statistically significant(P<0.05).Conclusion The selenium deficiency of myocardial cells leads to the decrease of mitochondrial STAT3 activity and mitochondrial function,thus promots the apoptosis of cardiomyocytes.

关 键 词：硒 肌细胞 心脏 线粒体 转录激活因子3 细胞凋亡 

分 类 号：R331.31[医药卫生—人体生理学]