高效阴离子交换色谱–脉冲安培法测定生态养护抑菌凝胶中的褐藻寡糖  

Determination of AOS in ecological conservation serum gel by HPAE–PAD

作　　者：成臻 成和颐 成源 CHENG Zhen;CHENG Heyi;CHENG Yuan(Metrology Testing Center,Xi’an North Opto-electronic Science and Technology Defense Co.,Ltd.,Xi’an 710043,China;Xi’an Gaoxin No.1 High School,Xi’an 710075,China;Xi’an ZKK Health Management Consulting Co.,Ltd.,Xi’an 710003,China)

机构地区：[1]西安北方光电科技防务有限公司检测计量中心,西安710043 [2]西安高新第一中学,西安710075 [3]西安祝康康健康管理咨询有限公司,西安710003

出　　处：《化学分析计量》2019年第6期43-47,共5页Chemical Analysis And Meterage

摘　　要：建立高效阴离子交换色谱-脉冲安培检测法测定生态养护抑菌凝胶中褐藻寡糖含量的方法。采用阴离子交换柱CarboPac PA10(250 mm×2 mm,3.5μm),以氢氧化钠、乙酸钠和水为洗脱液进行梯度洗脱,柱温为35℃,流量为0.8 mL/min。α-L-古罗糖醛酸单糖(PG)和β-D-甘露糖醛酸单糖(PM)的质量浓度分别在0.25~9.0μg/mL和0.18~8.4μg/mL范围内与色谱峰面积线性关系良好,线性相关系数分别为0.9997和0.9998,检出限分别为0.06,0.03μg/mL,定量限分别为0.20,0.10μg/mL。测定结果的相对标准偏差分别为1.11%,1.25%(n=6),加标回收率分别为101.03%和95.22%。该方法简便易行,灵敏度高,重现性好,适用于生态养护抑菌凝胶中褐藻寡糖的分析。The method for the determination of AOS in ecological conservation serum gel was established by high performance anion exchange chromatography with pulsed ampere detection(HPAE-PAD).The analysis was performed on a CarboPac PA10 column(250 mm×2 mm,3.5μm).With the gradient elution using NaOH,NaAc and H2O as the mobile phase.The column temperature was 35℃,and the flow rate was 0.8 mL/min.The mass concentration ofα-Lguluronic acid monosaccharide(PG)andβ-D-mannuronic acid monosaccharide(PM)had a good linear relationship with peak area in the range of 0.25-9.0μg/mL and 0.18-8.4μg/mL,respectively.The linear correlation coefficient was 0.9997 and 0.9998,the detection limit was 0.06,0.03μg/mL,the quantitative limit was 0.20,0.10μg/mL.The relative standard deriation of detection results of PG and PM was 1.11%and 1.25%(n=6),the recovery rate was 101.03%and 95.22%,respectively.The method was simple,sensitive and reproducible which is suitable for the analysis of alginate oligosaccharides in the ecological curing gel.

关 键 词：褐藻寡糖 α-L-古罗糖醛酸单糖 β-D-甘露糖醛酸单糖 生态养护抑菌凝胶 高效阴离子交换色谱-脉冲安培检测法 

分 类 号：O657.7[理学—分析化学]