GCM1在体外受精-胚胎移植胎盘及胚胎组织中表达及意义  

Expression and significance of GCM1 in in vitro fertilization-embryo transfer placenta and embryonic tissues

作　　者：郑秀丽[1] 刘静芳[1] 郑蓉[1] 王颖[2] 杨蕊[2] 张蕾 于丽[4] 张晗 赵亮 孙丽芳[1] ZHENG Xiu-li;LIU Jing-fang;ZHENG Rong;WANG Ying;YANG Rui;ZHANG Lei;YU Li;ZHANG Han;ZHAO Liang;SUN Li-fang(Department of Obstetrics and Gynecology Beijing Jishuitan Hospital,Beijing 100035,China;Department of Obstetrics and Gynecology,Peking University Third Hospital 100191,China;Department of Obstetrics and Gynecology,Beijing Tsinghua Chang Gung Hospital,Beijing 102218 China;Department of Obstetrics and Gynecology,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京积水潭医院,北京100035 [2]北京大学第三医院,北京100191 [3]北京清华长庚医院,北京102218 [4]北京大学北大医院,北京100034

出　　处：《中国优生与遗传杂志》2019年第10期1241-1244,F0003,共5页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金面上项目(81070493)

摘　　要：目的研究辅助生殖中体外受精-胚胎移植(IVF-ET)技术本身对早期胎盘绒毛和胚胎组织中GCM1及其基因表达影响。方法收集辅助生殖IVF-ET来源,双胚胎移植妊娠45-60天,经超声引导下减胎获得胎盘绒毛和胚胎组织各8例,作为研究组,对照组采用自然妊娠双胎45-60天人工流产中获得的胎盘绒毛和胚胎组织各10例。从采用免疫组织化学方法和实时荧光定量RT-PCR方法检测组织中GCM1蛋白和mRNA的表达及分布差异。结果(1)表达部位GCM1表达于胎盘绒毛细胞滋养层细胞和合体滋养层细胞的细胞膜和细胞质,广泛表达于胚胎各个胚层;(2)表达特点IVF-ET来源胎盘绒毛组织中GCM1表达光密度值显著低于自然妊娠组,差异有统计学意义。GCM1在IVF-ET和自然妊娠胚胎组织中表达无明显差异。(3)基因表达GCM1 mRNA在IVF-ET胎盘绒毛组织中低表达,而在胚胎组织中表达无显著性差异,与免疫组织化学结果相一致。结论GCM1蛋白和基因在IVF-ET胎盘绒毛组织中表达低于自然妊娠组织,而在胚胎组织中表达无明显差异,显示IVF-ET技术本身更加倾向于影响胎盘发育和功能,GCM1可能在IVF-ET相关疾病发病机制中发挥作用。Objective:To study the effect of IVF-ET on the expression of GCM1 and its gene in placenta villus and embryonic tissues.Methods:In vitro fertilization-embryo transfer patients were collected and transplanted 45-60 days after pregnancy by double embryo transfer.Ultrasound-guided embryo reduction was performed to obtain 8 cases of placental villus and embryonic tissue.As the study group,the control group used natural pregnancy twins 45-60 days abortion.Ten cases of placental villus and embryonic tissue were obtained.The expression and distribution of GCM1 protein and mRNA in tissues were detected by immunohistochemistry and real-time fluorescence quantitative RT-PCR.Results:1 Expression site.GCM1 is expressed in the cell membrane and cytoplasm of placental villous trophoblast cells and syncytiotrophoblasts,and GCM1 is widely expressed in embryonic germ layers;2 expression characteristics.The optical density of GCM1 expression in IVF-ET-derived placenta villi was significantly lower than that in natural pregnancy group.The difference was statistically significant.There was no significant difference in the expression of GCM1 between IVF-ET and natural pregnancy embryos.3 gene expression.GCM1 mRNA was lowly expressed in IVF-ET placenta villi,but there was no significant difference in embryonic tissue expression,consistent with immunohistochemical results.Conclusion:The expression of GCM1 protein and gene in IVF-ET placenta villi was lower than that in natural pregnancy tissues,but there was no significant difference in embryonic tissues.It was shown that IVF-ET itself is more likely to affect placental development and function.GCM1 may play a role in the pathogenesis of IVF-ET related diseases.

关 键 词：GCM1 体外受精-胚胎移植 发育 胎盘 胚胎 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]