烟草根部内生真菌群落结构和功能特征  

The structure and function of endophytic fungal community in tobacco root

作　　者：周家喜[1] 王茂胜 喻理飞[1,3] 刘京 邹晓[1] ZHOU Jia-Xi;WANG Mao-Sheng;YU Li-Fei;LIU Jing;ZOU Xiao(Department of Ecology/Institute of Fungus Resources,College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;College of Food and Pharmaceutical Engineering,Guizhou Institute of Technology,Guiyang,Guizhou 550003,China;Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),Guizhou University,Guiyang,Guizhou 550025,China;Guizhou Tobacco Company Zunyi Branch,Zunyi,Guizhou 563000,China)

机构地区：[1]贵州大学生命科学学院生态系/真菌资源研究所,贵州贵阳550025 [2]贵州理工学院食品药品制造工程学院,贵州贵阳550003 [3]贵州大学山地生态与农业生物工程协同创新中心,贵州贵阳550025 [4]贵州省烟草公司遵义市公司,贵州遵义563000

出　　处：《菌物学报》2019年第10期1610-1619,共10页Mycosystema

基　　金：贵州省生物学一流学科建设项目（GNYL[2017]009）;贵州省高等学校创新能力提升计划项目（黔教合协同创新字[2014]01）;贵州省烟草公司遵义市公司科技项目(2018-02)~~

摘　　要：烟草内生真菌是烟田生态系统的重要组成,根系是烟草内生真菌重要来源途径。本研究通过高通量测序技术分析了5个品种的烟草根部内生真菌群落结构及功能特征。结果表明,烟草根部内生真菌群落多样性较低;粪壳菌纲Sordariomycetes、座囊菌纲Dothideomycetes、散囊菌纲Eurotiomycetes、伞菌纲Agaricomycetes及锤舌菌纲Leotiomycetes为主要菌群,肉座菌目Hypocreales、煤炱目Capnodiales和散囊菌目Eurotiales为优势真菌目,丛赤壳科Nectriaceae、小戴维霉科Davidiellaceae和发菌科Trichocomaceae为优势真菌科,未定义腐生菌和动物病原菌是主要的生态功能群,但不同品种之间的真菌群落组成和生态功能分布差异显著。本研究为进一步挖掘利用烟草内生真菌资源,阐明烟草内生真菌的生态功能提供了参考。Tobacco endophytic fungi is an important component of the tobacco field ecosystem.Roots are important source of tobacco fungal endophytes.The community structure and functional characteristics of the endophytic fungi from roots of five varieties of tobacco were analyzed by high-throughput sequencing.The results showed that the diversity and richness of endophytic fungal community in the tobacco roots were low.Sordariomycetes,Dothideomycetes,Eurotiomycetes,Agaricomycetes and Leotiomycetes were the main fungal groups.Hypocreales,Capnodiales and Eurotiales were dominant order,and Nectriaceae,Davidiellaceae and Trichocomaceae were dominant family.Undefined saprophytes and animal pathogens were the main ecological functional groups.However,the distribution of community composition and ecological function were significantly different between tobacco varieties.

关 键 词：内生真菌 多样性 高通量测序 

分 类 号：S57[农业科学—作物学]