蝉棒束孢（蝉花）不同提取物抑制小鼠肥胖的比较  

Comparative analyses of different solvent extracts of Isaria cicadae on obesity control using a mouse model

作　　者：王东东[1] 祝响响 高亮 陈地灵[1] WANG Dong-Dong;ZHU Xiang-Xiang;GAO Liang;CHEN Di-Ling(Guangdong Institute of Microbiology,Guangdong Academy of Sciences,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangzhou,Guangdong 510070,China)

机构地区：[1]广东省微生物研究所广东省科学院华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室

出　　处：《菌物学报》2019年第10期1689-1701,共13页Mycosystema

基　　金：广东省科学院发展专项资金（2018GDASCX-0102）;广州市科技计划(201707020022)~~

摘　　要：蝉棒束孢(蝉花)Isariacicadae是药食两用真菌,具有多种生理活性,但其物质基础依然不明确。据此,本实验采用长期高脂高胆固醇饮食制备肥胖小鼠模型,灌胃给予蝉棒束孢乙醇提取物、水提取物和氢氧化钠碱水提取物等不同提取物,测定其血清中总胆固醇(T-CHO)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)等脂质代谢指标;观察其肝、肠组织的病理变化情况,以及肠道内容物中的菌群结构等,综合分析并筛选出蝉棒束孢改善肥胖的有效提取物或成分,为其进一步的深加工提供基础。结果表明,蝉棒束孢的不同溶剂提取物均可不同程度减缓高脂高胆固醇饮食诱导小鼠肥胖,同时改善其脂质代谢情况,减轻脂肪在肝脏中的堆积,减缓肠组织损伤,并调整肠道菌群结构,蝉棒束孢醇提取物效果较优,但其具体作用机制还有待进一步的研究揭示。This study aims at screening the active extract from Isaria cicadae for controlling obesity and its complications. An obesity mouse model was prepared by feeding high fat and cholesterol diet to mice. Isaria cicadae extracts(ethyl alcohol extract, aqua destillata extract and alkaline water extract) were fed to the mice for 35 days by gavage. Histopathological changes of liver and colon, serum levels of total cholesterol(T-CHO), total triglycerides(TG), low-density lipoprotein cholesterol(LDL-C) and high-density lipoproteincholesterol(HDL-C) and gut microbiota were monitored. Results showed that all of the three extractions could reduce the body weight and improve the serum levels of T-CHO, TG, LDL-C and HDL-C of the experimental mice. The pathological observation revealed that the damages of liver and colon tissues induced by high fat and cholesterol diet were ameliorated more or less, and the alpha and beta diversity and gut microbiota composition were also improved. The ethyl alcohol extract of Isaria cicadae(ICE) has the best effect among the three tested samples, indicating that ICE would be the most active extract for obesity control while the functional mechanism remains to be studied.

关 键 词：高脂高胆固醇饮食 肠道菌群 肠道稳态 肠道损伤 代谢调控 

分 类 号：R28[医药卫生—中药学]