基于微卫星标记的圆口铜鱼亲子鉴定技术  

PARENTAGE ANALYSIS OF COREIUS GUICHENOTI USING MICROSATELLITES

作　　者：何勇凤[1] 朱永久[1] 吴兴兵[1] 龚进玲 杨德国[1] HE Yong-Feng;ZHU Yong-Jiu;WU Xing-Bing;GONG Jin-Ling;YANG De-Guo(Key Laboratory of Freshwater Biodiversity Conservation,Ministry of Agriculture and Rural Affairs of China,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223)

机构地区：[1]中国水产科学研究院长江水产研究所农业农村部淡水生物多样性保护重点实验室

出　　处：《水生生物学报》2019年第6期1216-1223,共8页Acta Hydrobiologica Sinica

基　　金：中国长江三峡集团公司项目(JGJ/025 2013)资助~~

摘　　要：为快速有效地鉴别不同的圆口铜鱼家系及来源,研究从已发表的40个微卫星标记中筛选出20个多态性较高且稳定扩增的微卫星位点,通过对8个圆口铜鱼家系339尾个体进行微卫星基因分型检测,建立了圆口铜鱼荧光微卫星标记与多重毛细管电泳相结合的亲子鉴定技术。遗传多样性分析结果显示,圆口铜鱼8个家系群体的平均等位基因数(Na)为9个,平均多态信息含量(PIC)为0.616,平均期望杂合度(He)为0.659,平均观测杂合度(Ho)为0.691,其中子一代群体的遗传多样性水平明显低于亲本群体。亲子鉴定分析结果显示,当双亲基因型未知时其单亲累积排除概率(CE-1P)为0.99954473,当单亲基因型已知时其累积排除概率(CE-2P)为0.99999825,当双亲基因型未知时其双亲累积排除概率(CE-PP)为1.00000000,当使用20个微卫星位点进行亲子鉴定时,297尾子一代均能正确找到其父母本,亲子鉴定准确率为100%。由此可见,研究建立的圆口铜鱼亲子鉴定技术是可靠的,能为圆口铜鱼的家系管理、种群遗传管理和增殖放流效果评估提供科学依据.To quickly and effectively distinguish different families and pedigrees of Coreius guichenoti,the present study screened twenty microsatellite loci with high polymorphism and stable amplification from the reported forty microsatellite loci using 339 individuals from eight families of C.guichenoti.Through this study,a paternity technology combined with fluorescent microsatellites and multiple capillary electrophoresis in C.guichenoti were established.Genetic diversity analysis revealed that the average allele number(Na)of eight families of C.guichenoti,the average polymorphism information content(PIC),the average expected heterozygosity(He),and the average observed heterozygosity(Ho)were 9,0.616,0.659,and 0.691,respectively.Genetic diversity indices of the offspring group were significantly lower than those of the parent group.Parentage analysis revealed that the combined exclusion probability of a first candidate parent given only the genotype of the offspring(CE-1P),a second candidate parent given the genotype of the offspring and one known parent(CE-2P)and a candidate parent poor give only the genotype of the offspring(CE-PP)were 0.99954473,0.99999825 and 1.00000000,respectively,indicating that the accurate rate of parentage assignment was 100%.Thus we established a reliable paternity technology of C.guichenoti,which can provide scientific basis for its genealogical management,population genetic management,and assessment of stock enhancement effect.

关 键 词：圆口铜鱼 微卫星 亲子鉴定 

分 类 号：Q173[生物学—水生生物学]