金匮肾气汤对单侧输尿管梗阻大鼠肾纤维化缺氧的影响及作用机制  

Effects of Jingui Shenqi Decoction on hypoxia due to renal fibrosis in rats and its mechanism

作　　者：刘春燕[1] 梁承谦 谷依红 易纯[1] 唐群[1] Liu Chunyan;Liang Chengqian;Gu Yihong;Yi Chun;Tang Qun(Hunan University of Chinese Medicine,Hunan 410208,China)

机构地区：[1]湖南中医药大学

出　　处：《北京中医药大学学报》2019年第9期753-759,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(No.81603470);湖南中医药大学一级学科基础医学建设项目~~

摘　　要：目的探讨金匮肾气汤对单侧输尿管梗阻(UUO)大鼠肾间质纤维化缺氧的影响及作用机制。方法雄性SD大鼠24只,随机分为假手术组、模型组、金匮肾气汤组、依那普利组,每组6只,采用左侧输尿管结扎术造模,UUO术后第1天开始灌胃,金匮肾气汤1.8 g/kg,依那普利10 mg/kg,每日1次。第21天处死大鼠,取梗阻肾组织,HE和Masson染色观察肾组织病理改变,免疫荧光检测Tomato Lectin、Hypoxyprobe-1和缺氧诱导因子-1α(HIF-1α)在肾组织的表达, Western Blot检测转化生长因子-β1 (TGF-β1)、骨形态发生蛋白(BMP-7)和HIF-1α在肾组织的表达。结果与模型组比较,金匮肾气汤治疗后,HE和Masson染色结果提示梗阻肾肾盂扩张减轻、肾实质变厚,间质胶原阳性面积减少,Western Blot检测梗阻肾组织中TGF-β1蛋白表达下调、BMP-7蛋白表达上调(P<0.05);免疫荧光检测显示梗阻肾组织中血管密度增加、缺氧改善,HIF-1α表达下调(P<0.05)。结论金匮肾气汤可以减轻UUO大鼠梗阻肾组织纤维化,其作用机制可能与其改善肾间质缺氧有关。Objective To investigate the effect of Jingui Shenqi(Golden Cabinet’s Kidney Qi) Decoction on hypoxia in unilateral ureteral obstruction(UUO) rats and its mechanism. Methods 24 male SD rats were randomly divided into 4 groups: sham operated group, UUO model group, Jingui Shenqi Decoction(JSD) treatment group, and enalapril(ENP) treatment group with 6 rats in each group. The models were established by left ureter ligation. From the first day after UUO, the JSD group and ENP group were given intragastrically JSD(1.8 g/kg) and enalapril(10 mg/kg) respectively once a day. After intervention for 21 days, the rats were executed. The obstructed kidney tissues were taken and stained with HE and Masson to observe pathological changes. The expressions of Tomato Lectin, Hypoxyprobe-1 and HIF-1α were detected by immunofluorescent analysis. The protein expressions of TGF-β1, BMP-7 and HIF-1α in renal tissue were detected with Western blot. Results Compared with UUO model group, after treatment with JSD, HE and Mason staining showed that obstructive pyelomegaly was reduced, renal parenchyma became thicker, and interstitial collagen positive area was reduced. Western Blot assay showed that the protein expression of TGF-β1 decreased and that of BMP-7 increased in obstructed kidney tissues(P< 0.05). Immunofluorescence assay showed that vascular density increased, hypoxia improved, and HIF-1α expression decreased in obstructed kidney tissue(P< 0.05). Conclusion JSD can alleviate renal fibrosis in UUO rats, which may be related to its effect of ameliorating renal interstitial hypoxia.

关 键 词：金匮肾气汤 肾纤维化 单侧输尿管梗阻大鼠 缺氧 缺氧诱导因子-1Α 

分 类 号：R285.5[医药卫生—中药学]