光照和周期性射频辐射对果蝇睡眠影响的研究  

Effects of light and radiofrequency electromagnetic fields on sleep of Drosophila melanogaster

作　　者：李志辉 张紫燕[1,3] 张虹影 王亚红 章路 唐超 廖艳艳[1,3] 蔡澎 LI Zhi hui;ZHANG Zi yan;ZHANG Hong ying;WANG Ya hong;ZHANG Lu;TANG Chao;LIAO Yan yan;CAI Peng(Key Lab of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen,Fujian 361021,China;College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100190,China;Xiamen Key Laboratory of Physical Environment,Xiamen,Fujian 361021,China;Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China)

机构地区：[1]中国科学院城市环境研究所环境与健康重点实验室,福建厦门361021 [2]中国科学院大学资源与环境学院,北京100190 [3]厦门市物理环境重点实验室,福建厦门361021 [4]中国科学院上海生命科学研究院,上海200031

出　　处：《军事医学》2019年第5期357-363,共7页Military Medical Sciences

基　　金：中国科学院重点部署项目（KGFZD-135-16-024）;国家电网公司科技项目（GY71-16-008）;厦门市科技计划项目（3502Z20172028）

摘　　要：目的探究周期性射频辐射(RF)对果蝇睡眠行为的影响及调控机制。方法实时连续监测果蝇在光照、RF(1800 MHz,1 W/m2,12 h/d)及黑暗条件下的行为变化,对比分析各条件下果蝇运动和睡眠的异同。收集处理2 h后的样品,分析RF对果蝇生物钟基因per、tim、clk、cyc和cry及神经递质关键合酶编码基因hdc、gdh、gad和trh表达水平的影响。结果光照组和RF组的运动和睡眠均表现出与处理周期一致的节律变化,黑暗组节律振幅减弱;与黑暗组相比,光照组果蝇白天和夜晚的睡眠质量均显著上升,RF组果蝇白天和黑暗组的表现一致,但夜间睡眠时间显著延长,睡眠质量上升。短时间暴露下,光照组和RF组果蝇上述5个生物钟基因表达水平全部下调,只是光照组clk、RF组tim下调更显著。此外,光照组和RF组hdc和gad基因表达水平上调,gdh下调,RF组trh基因下调。结论与光同步的周期性RF可能通过影响生物钟和神经递质相关合成基因的表达,进而影响果蝇的睡眠节律、时间及质量,在昼夜节律调控方面可能具有类似光照的功能。Objective To investigate the effect of periodic radiofrequency(RF)radiation on sleep behavior of Drosophi la melanogaster and the regulatory mechanism.Methods The behavioral changes of D.melanogaster under light,RF radi ation(1800 MHz,1 W/m^2,12 h/d)and dark conditions were continuously monitored in real time.The similarities and dif ferences between D.melanogaster movement and sleep under different conditions were compared and analyzed.Samples were collected for 2 h to analyze the effects of RF radiation on the expression levels of clock genes,such as per,tim,clk,cyc and cry,and the neurotransmitter key synthase encoding genes hdc,gdh,gad and trh.Results The movement and sleep of the illumination group and the RF group showed rhythm changes consistent with the treatment cycle,and the rhythm amplitude in the dark group was weakened.Compared with the dark group,the sleep quality of the light group in creased significantly during the day and night.As for the RF group,the performance of D.melanogaster during the day was consistent with that of the dark group,but the nighttime sleep time was significantly prolonged and the quality of sleep im proved.Under the short term exposure,the expression levels of the above five clock genes in the light and RF groups were all down regulated except that the clk and tim were down regulated to different extents.In addition,the expression levels of hdc and gad genes were up regulated in the light and RF groups,gdh genes were up regulated,and the expression levels of trh gene was down regulated in the RF group.Conclusion Periodic RF radiation synchronized with light may affect the expression of circadian clock and neurotransmitter related synthetic genes,hereby affecting the rhythm,time and quality of sleep of the D.melanogaster,and may perform a similar illumination function in the regulation of circadian rhythm.

关 键 词：辐射 光照期 射频电磁场 果蝇 睡眠 神经递质 生物钟 

分 类 号：R338.6[医药卫生—人体生理学] R14[医药卫生—基础医学]