凝集素样氧化型低密度脂蛋白受体-1在人巨细胞病毒感染致内皮细胞损伤中的作用  

The role of lectin-like Ox-LDL receptor-1 on endothelial cell injury caused by human cytomegalovirus infection

作　　者：车晶晶[1] 王会奇 杜艺彤 许晓娇 易立[1] 王瑞金[1] 陈君[1] 刘磊[3] 王得新[1] 脱厚珍[1] Che Jingjing;Wang Huiqi;Du Yitong;Xu Xiaojiao;Yi Li;Wang Ruijin;Chen Jun;Liu Lei;Wang Dexin;Tuo Houzhen(Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beijing 100150,China;Neurological Disease Rehabilitation Center,Beijing Rehabilitation Hospital,Capital Medical University,Beijing 100041,China;Department of Neurology,Tongren Hospital,Capital Medical University,Beijing 100730,China)

机构地区：[1]首都医科大学附属北京友谊医院神经内科,北京100150 [2]首都医科大学附属北京康复医院神经疾病康复中心,北京100041 [3]首都医科大学附属同仁医院神经内科,北京100730

出　　处：《中华实验和临床病毒学杂志》2019年第5期500-503,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金(81241039)。

摘　　要：目的初步探讨凝集素样氧化型低密度脂蛋白受体-1(lectin-like Ox-LDL receptor-1,LOX-1)在巨细胞病毒(cytomegalovirus,CMV)感染致内皮细胞损伤的作用机制。方法体外培养人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC),以人巨细胞病毒(human cytomegalovirus,HCMV)AD169株感染HUVEC,设置HCMV组、对照组、角叉菜胶组、HCMV+角叉菜胶组,通过实时定量PCR(real-time PCR)方法检测LOX-1及粘附分子ICAM-1和VCAM-1 mRNA的表达情况,通过硝酸还原酶法检测细胞外一氧化氮(NO)含量的变化,然后比较各组上述指标的变化情况。结果HUVEC感染HCMV后24 h,HCMV组LOX-1、ICAM-1和VCAM-1 mRNA的表达水平较对照组明显增加,分别为1.649±0.078和1.000±0.086,1.751±0.041和1.000±0.048,1.634±0.072,1.000±0.102,两组间差异具有统计学意义(P<0.05);HCMV组细胞培养上清中NO含量也较对照组增加,为121.27±1.93和106.79±2.266,两组间差异均具有统计学意义(P<0.01)。加入LOX-1抑制剂角叉菜胶后,ICAM-1、VCAM-1 mRNA表达水平以及细胞培养上清中NO的含量均较HCMV组明显下降,各组间差异具有统计学意义(P<0.05)。结论HCMV感染能够上调HUVEC LOX-1及粘附分子的表达,同时也能促进细胞外NO生成,LOX-1可能在HCMV感染致内皮细胞氧化应激活动增强及血管内皮细胞损伤中起重要的介导作用。Objective To investigate the role of lectin-like Ox-LDL receptor-1(LOX-1)in the activation and oxidative stress of cultured human umbilical vein endothelial cell(HUVEC)after human cytomegalovirus(HCMV)infection.Methods HUVEC were divided into four groups:HCMV,Control,Carrageenan,and HCMV+Carrageenan.After HCMV AD169 infection,the supernatant of the culture was extracted,and cells were lysed.The levels of LOX-1 mRNA,intercellular adhesion molecule-1(ICAM-1)mRNA and vascular cellular adhesion molecule-1(VCAM-1)in HUVEC were measured by real-time PCR.And the content of nitrogen monoxidum(NO)of the supernatant was detected by nitrate reductase method accordingly.Results 24 h after infection,the mRNA expression of LOX-1,ICAM-1 and VCAM-1 in HUVEC of HCMV infected group increased obviously compared to control,and NO quantity increased accordingly.The mRNA expression of LOX-1,ICAM-1 and VCAM-1 and the quantity of NO decreased after adding the LOX-1 inhibitor carrageenan.There was significant difference between groups(P<0.05).Conclusions HCMV may increase the mRNA expression of ICAM-1 and VCAM-1 and quantity of NO by upregulating the mRNA expresion of LOX-1,which may contribute to the formation of a therosclerosis(AS).

关 键 词：动脉粥样硬化 人巨细胞病毒 凝集素样氧化型低密度脂蛋白受体-1 氧化应激 血管内皮粘附分子 脐静脉内皮细胞 

分 类 号：R54[医药卫生—心血管疾病]