分子溯源技术在1例艾滋病感染来源分析中的应用  

Application of molecular tracing technique in one analysis of AIDS infection source

作　　者：许文平[1] 余明 孔丹 肖体呈 邹家凤[1] 徐立新 刘小红 Xu Wenping;Yu Ming;Kong Dan;Xiao Ticheng;Zou Jiafeng;Xu Lixin;Liu Xiaohong(Luzhou Center for Disease Control and Prevention,Luzhou 646600,China)

机构地区：[1]泸州市疾病预防控制中心,646600

出　　处：《中华实验和临床病毒学杂志》2019年第5期526-529,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的利用分子溯源技术,推断1例13岁白血病患者Wei感染HIV的来源。方法采集与HIV阳性献血员Lang有关联供求关系的3份献血者和受血者样本,进行病毒载量检测和pol基因测序鉴定,对pol基因进行分析,同时利用HyPhy2.2.4和Cytoscape3.6.1将3份样本序列与泸州市获得的其他HIV同亚型序列构建分子网络。结果3人的HIV-1病毒载量检测结果为:献血员Lang 933 cps/ml(已治疗),受血人Wei 89813 cps/ml,受血人Deng 85158 cps/ml。3份样本为HIV CRF01_AE重组亚型,pol基因片段同源性分别为:98.8%(Lang与Wei)、99.7%(Lang与Deng)、99.1%(Wei与Deng),3份样本基因序列连接成簇(基因距离小于0.004)。结论白血病患者Wei系HIV-1窗口期输血感染,3人感染同一HIV-1毒株可能性较大。采供血机构普及使用高灵敏度核酸检测方法,可有效提高用血安全。Objective To determine the HIV infection of a 13-year-old leukemia patient Wei using molecular tracing technique.Methods Three blood samples were collected from the persons who were associated with HIV positive blood donation member Lang.The viral load was tested and pol gene was sequenced and analyzed.At the same time using HyPhy2.2.4 and Cytoscape 3.6.1 to establish the molecular network with these 3 samples sequences and other HIV subtypes sequences obtained from Luzhou.Results The HIV-1 viral load test result of the three patients were:Lang 933 CPS/ml(treated,blood donor),Wei(blood recipitent)89813 CPS/ml,Deng(blood recipitent)85158 CPS/ml.The subtype of HIV-1 of these three samples were all recombinant HIV CRF01_AE,and the pol gene similarity was 98.8%(Lang and Wei),99.7%(Lang and Deng),and 99.1%(Wei and Deng),respectively.The gene sequences of the three samples were linked into clusters(the gene distance was less than 0.004).Conclusions The patient Wei with leukemia was infected with HIV-1 due to blood transfusion during the seroconversion period,and the three patients were more likely to be infected with the same HIV-1 strain.It is necessary to popularize the use of high-sensitivity nucleic acid detection method in blood collection and supply institutions,which can effectively improve the safety of blood use.

关 键 词：HIV-1 病毒载量 基因 窗口期 

分 类 号：R51[医药卫生—内科学]