哈尔滨市腹泻患者星状病毒感染的分子流行病学研究  

Genetic characteristics of human astrovirus isolated from patients with diarrhea,Harbin

作　　者：石鑫[1] 许军[1] 舒畅[1] 冷焱 曹博[1] 华华[1] SHI Xin;XU Jun;SHU Chang;LENG Yan;CAO Bo;HUA Hua(Heilongjiang Provincial Center for Disease Control and Prevention,Harbin,Heihongjiang150030,China)

机构地区：[1]黑龙江省疾病预防控制中心,黑龙江哈尔滨150030 [2]鸡西市疾病预防控制中心,黑龙江鸡西158100

出　　处：《现代预防医学》2019年第21期3841-3844,共4页Modern Preventive Medicine

基　　金：国家科技艾滋病和病毒性肝炎等重大传染病防治辽宁及周边省市传染病病原谱流行规律研究课题(黑龙江省特色传染病病原谱流行规律研究子课题)(编号:2017ZX10103007-004)

摘　　要：目的了解黑龙江省哈尔滨市腹泻患者人星状病毒(HAstV)感染情况,并分析其分子流行病学特征。方法2017年1月-2018年12月共采集340份哨点医院腹泻病人的粪便标本,采用Real-time PCR进行HAstV检测,对HAstV阳性标本采用RT-PCR方法扩增ORF2区,对PCR产物测序并进行系统进化分析。结果HAstV阳性检出率为6.18%(21/340),男性阳性检出率为8.52%(15/176),女性阳性检出率为3.66%(6/164),差异无统计学意义(χ2=3.466,P=0.063)。HAstV感染阳性的患者年龄主要集中在2岁以下年龄组及3~5岁年龄组,检出率分别为10.71%(18/168)、10.53%(2/19);季节高峰在2月,检出率为18.18%(2/11)。对阳性标本进行测序,获得16株序列,系统进化分析结果显示,11株HAstV-1,且均为1a型;4株HAstV-5型;1株HAstV-4c型。结论HAstV是哈尔滨市病毒性腹泻的重要病原之一,且存在多种基因型,HAstV-1型是主要流行株。HAstV-1a型及HAstV-4c型是在黑龙江省哈尔滨市首次发现及报道的型别。Objective To investigate the infection of human astrovirus(HAstV)in patients with diarrhea in Harbin,Heilongjiang Province,and to study its molecular epidemiological characteristics.Methods From January 2017 to December 2018,a total of 340 fecal specimens from patients with diarrhea in sentinel hospitals were collected.Real-time PCR was used for HAstV detection.The HAstV positive samples were amplified by RT-PCR.The PCR products were sequenced,and phylogenetic analyses were carried out.Results The positive rate of HAstV was 6.18%(21/340),the positive rate of male detection was 8.52%(15/176),and the positive rate of female detection was 3.66%(6/164).There was no statistically significant difference(χ2=3.466,P=0.063).The age of patients with positive HAstV infection was mainly in the age groups under 2 years old and the age group 3 to 5 years old.The detection rates were 10.71%(18/168)and 10.53%(2/19),respectively.The peak season was in February.The detection rate was 18.18%(2/11).The positive samples were sequenced and 16 sequences were obtained.The phylogenetic analysis showed that 11 strains were HAstV-1a,4 strains were HAstV-5,and 1 strain was HAstV-4c.Conclusion HAstV is one of the important pathogens of viral diarrhea in Harbin,and there are many genotypes.HAstV-1 is the predominant strain.HAstV-1a and HAstV-4c are the first types discovered and reported in Harbin,Heilongjiang Province.

关 键 词：人星状病毒 流行病学 基因型 

分 类 号：R181.3[医药卫生—流行病学]