阿希蕉合生花组培快繁技术研究  

Tissue Culture and Rapid Propagation Technology of Hopson Flower of Musa velutina

作　　者：黄东梅[1] 许奕[1] 李敬阳[1] 李羽佳[1] 林妃[1] 李艳霞[1] 宋顺[1] HUANG Dongmei;XU Yi;LI Jingyang;LI Yujia;LIN Fei;LI Yanxia;SONG Shun(Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Banana Genetic Improvement,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院海口实验站/海南省香蕉遗传改良重点实验室

出　　处：《热带作物学报》2019年第11期2174-2177,共4页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划子课题-柑桔、香蕉等(亚)热带果树优质高效生产关键技术研究与示范项目(No.2014BAD16B01);现代农业产业技术体系专项资金项目(No.CARS-31-02);国家自然科学基金项目(No.31501371)

摘　　要：阿希蕉为野生香蕉资源,可作为重要的遗传育种资源并具有观赏价值。本研究以阿希蕉合生花为植物材料,进行组培快繁技术研究。结果表明,阿希蕉在巴西蕉愈伤诱导培养基上可诱导出愈伤组织,最佳芽分化培养基配方为MS+6-BA(3.0 mg/L)+NAA(0.1~0.2 mg/L),最佳芽增殖培养基配方为MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L),最佳生根培养基配方为MS+IBA(3.0 mg/L)+NAA(0.3 mg/L)。从取材到获得根系完整的组培苗约需100 d,繁殖效率100株/花苞以上,增殖效率高,可达到快速繁育的目的。As a wild banana resource,Musa velutina can be regarded as an important genetic and breeding resource and has an ornamental value.In this study,the tissue culture and rapid propagation technology of Musa velutina were studied using the hopson flowers as the plant materials.Callus could be induced by the callus induction medium.The best bud differentiation medium was MS+6-BA(3.0 mg/L)+NAA(0.1-0.2 mg/L),the best bud proliferation medium was MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L),and the best rooting medium was MS+IBA(3.0 mg/L)+NAA(0.3 mg/L).It took about 100 d from sampling to obtaining tissue culture seedlings with complete root system,and the reproduction efficiency was more than 100 plants/flower bud.

关 键 词：阿希蕉 合生花 组培 芽分化 芽增殖 

分 类 号：S686[农业科学—观赏园艺]