游泳运动对异育银鲫“中科3号”甲状腺激素代谢的影响  

Effect of swimming on the metabolism of thyroid hormones of Carassius auratus gibelio var.CAS Ⅲ

作　　者：王海珊 李长江 汤蓉[1] 李大鹏[1] 梁骁 熊梅 Kommaly Onxayvieng 李莉[1] WANG Hai-shan;LI Chang-jiang;TANG Rong;LI Da-peng;LIANG Xiao;XIONG Mei;LI Li(College of Fisheries,Huazhong Agricultural University/National Demonstration Center for Experimental Aquaculture Education/Hubei Provincial Engineering Laboratory for Pond Aquaculture,Wuhan 430070,China;Yichang Aquatic Technology Extension Station,Yichang 443003,Hubei,China)

机构地区：[1]华中农业大学水产学院,水产养殖国家级实验教学示范中心,池塘健康养殖湖北省工程实验室,武汉430070 [2]宜昌市水产技术推广站,湖北宜昌443003 [3]Department of Livestock and Fisheries,Ministry of Agriculture and Forestry,Vientiane,Lao PDR

出　　处：《淡水渔业》2019年第6期3-9,26,共8页Freshwater Fisheries

基　　金：现代农业产业技术体系建设专项资金(CARS-45);国家自然科学基金(31502140);中央高校基本科研业务费专项资金资助项目(2662015PY119)

摘　　要：实验以异育银鲫“中科3号”(Carassius auratus gibelio var.CASⅢ)作为研究对象,以体长/秒(bl/s)为流速单位设置0、0.5、1和2 bl/s 4个水流速度实验组对鱼类进行游泳训练。运动训练8周后,检测其血清皮质醇含量、甲状腺激素(THs)和促甲状腺素(TSH)含量、肝脏脱碘酶(IDs)活性、甲状腺组织形态以及与甲状腺激素代谢调控相关基因的表达量,以此探讨游泳运动对异育银鲫甲状腺激素代谢的影响。结果显示,游泳运动未引起血清皮质醇的显著变化,但血清四碘甲腺原氨酸(T4)与三碘甲腺原氨酸(T3)含量上升,在0.5 bl/s运动组中出现显著上升,游离四碘甲腺原氨酸(FT4)含量在2 bl/s运动组显著性上升,游离三碘甲腺原氨酸(FT3)含量在对照组与三个运动组间不存在显著性差异。TSH水平在0.5 bl/s与1 bl/s运动组中显著性上升。Ⅰ型脱碘酶(ID1)活性在0.5 bl/s运动组显著上升;Ⅱ型脱碘酶(ID2)活性随着流速的增加呈逐渐上升趋势,但各个实验组间无显著性差异;Ⅲ型脱碘酶(ID3)活性随着流速的上升而显著性下降。8周的运动训练显著性提升了甲状腺激素受体(tr-α)的基因表达水平。各组实验鱼的甲状腺腺泡胶质均匀,但各训练组的腺泡上皮细胞核有显著增大现象。研究结果表明,游泳运动可通过下丘脑-垂体-甲状腺轴调控甲状腺激素代谢过程,提升外周甲状腺激素水平。Four experimental groups including 0,0.5,1 and 2 bl/s respectively were set with body length(bl)/s as the velocity unit for swimming training to explore the effect of swimming on thyroid hormone metabolism of Carassius auratus gibelio var.CASⅢ.After 8 weeks of training,we analysed the serum cortisol content,thyroid hormone content and thyroid-stimulating hormone(TSH)content,liver thyroid deiodinase activity,thyroid histological morphology and the expression of genes related to thyroid hormone metabolism regulation.The results showed that swimming did not cause significant changes in serum cortisol(P>0.05),which indicated that fish could adapt to the swimming lower than 2 bl/s water velocity.And then,after 8 weeks of swimming the levels of serum 3,5,3′-Triiodothyronine(T4)and Thyroxine(T3)increased in the swimming group,especially in the 0.5 bl/s grouP.The content of Free thyroxine(FT4)increased significantly in the 2 bl/s group than the other three groups.There was no significant difference in the content of Free triiodothyronine(FT3)between the control group and the three swimming groups.The levels of serum TSH increased significantly in either 0.5 bl/s or 1 bl/s groups.The thyroid acinar glia of the fish was uniform,but the acinar epithelial nuclei in the swimming group were enlarged.In addition,compared with the control group,the activity of type 1 iodothyronine deiodinase(ID1)increased significantly in the 0.5 bl/s group,the activity of type 2 iodothyronine deiodinase(ID2)increased gradually,but there was no significant difference among four groups,and the activity of type 3 iodothyronine deiodinase(ID3)decreased significantly with the increase of water velocity.Swimming significantly increased the gene expression of thyroid hormone recePtor(tr-α).The thyroid follicle ePithelial gelatin in each group of fish was uniform,but the nucleus of follicle epithelial cells was increased significantly in swimming groups.The results showed that swimming could regulate thyroid hormone metabolism through hypothalamus

关 键 词：异育银鲫“中科3号”(Carassius auratus GIBELIO var.CASⅢ) 甲状腺激素 脱碘酶 基因表达 

分 类 号：S917.4[农业科学—水产科学]