稻田养殖的大鳞副泥鳅出血病病原菌的分离鉴定与药敏试验  

Isolation,identification and antimicrobial susceptibility test of pathogenic bacteria of hemorrhagic disease from Paramisgurnus dabryanus cultured in the rice field

作　　者：雷坤 谢业扬 马远雄 王邦杰 范道周 陆专灵 徐娣美 何有柯 韦友传[1] LEI Kun;XIE Ye-yang;MA Yuan-xiong;WANG Bang-jie;FAN Dao-zhou;LU Zhuan-ling;XU Di-mei;HE You-ke;WEI You-chuan(College of Animal Science and Technology,Guangxi University,Nanning 530000,China;Guangxi Academy of Fishery Sciences/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning 530021,China)

机构地区：[1]广西大学动物科学技术学院,南宁530000 [2]广西壮族自治区水产科学研究院,广西水产遗传育种与健康养殖重点实验室,南宁530000

出　　处：《淡水渔业》2019年第6期70-74,共5页Freshwater Fisheries

摘　　要：为确定稻田养殖的大鳞副泥鳅(Paramisgurnus dabryanus)出血病病原与防治措施,应用微生物分离技术和分子生物学相关方法,对患出血病的大鳞副泥鳅进行病原菌的分离和鉴定并分析其药物敏感性。从患出血病大鳞副泥鳅中分离到3株优势菌(命名为DLFNQ-1、DLFNQ-2、DLFNQ-3);回归感染实验结果显示,仅DLFNQ-1对大鳞副泥鳅具有致病性,半致死浓度为2.3×10^7 CFU/mL;生理生化试验表明,DLFNQ-1菌株对葡萄糖、阿拉伯糖、氧化酶、七叶灵等反应为阳性,对柠檬酸、尿素酶等反应为阴性,与嗜水气单胞菌的生理生化特性相符;DLFNQ-1菌株的16S rDNA序列与嗜水气单胞菌(JN391411.1)的相似度达到99.7%,N-J进化树显示其16S rDNA与其它嗜水气单胞菌的聚为一支。DLFNQ-1菌株对庆大霉素、妥布霉素这两种抗生素高度敏感,对阿洛西林、哌拉西林、卡那霉素等中度敏感,对青霉素、阿莫西林、四环素等呈现出一定的耐药性。结果表明,引起稻田养殖大鳞副泥鳅出血病的病原为嗜水气单胞菌(Aeromonas hydrophila),稻田养殖时可选用庆大霉素、妥布霉素进行防控。The pathogenic strains were isolated and identified using physiological,biochemical and molecular biological methods,and antimicrobial susceptibility characteristics of the pathogen were analysed to explore the pathogens and control measures for Paramisgurnus dabryanus cultured in the rice field with a hemorrhagic disease.In this study,three bacterial(named DLFNQ-1、DLFNQ-2、DLFNQ-3)were isolated from P.dabryanus with a hemorrhagic disease.Artificial infection results showed that only DLFNQ-1 had active pathogenicity to P.dabryanus,and the semi-lethal dose was 2.3×107^ CFU/mL.Physiological and biochemical tests showed DLFNQ-1 strain was positive for glucose,arabinose,oxidase and esculetin reactions,while was negative for citric acid,urease and MR reactions,which were consistent with these of Aeromonas hydrophila.DLFNQ-1 strain 16S rDNA sequence shared 99.7% homology with that of A.hydrophila(JN391411.1).The N-J phylogenetic tree analysis showed that DLFNQ-116S rDNA were clustered with that of A.hydrophila.DLFNQ-1 strain is highly sensitive to gentamicin and tobramycin,moderately sensitive to azlocillin,piperacillin and kanamycin,and resistance to penicillin,amoxicillin and tetracycline.The pathogen causing hemorrhagic disease of P.dabryanus is A.hydrophila.When breeding,gentamicin and tobramycin could be selected for prevention and control.

关 键 词：大鳞副泥鳅(Paramisgurnus dabryanus) 出血病 嗜水气单胞菌(Aeromonas hydrophila) 药敏试验 

分 类 号：S941.42[农业科学—水产养殖]