胃复方对人胃癌BGC-823细胞移植瘤裸鼠作用及其对c-Myc、人端粒酶逆转录酶基因表达的影响  

Effects of Weifu Formula on Nude Mice with Human Gastric Cancer BGC-823 Cell Xenograft Tumor and Its Effects on Expression of C-Myc and h TERT Genes

作　　者：张顺荣 李东芳[2] 何寄琴 焦蕉[2] 李玉明[2] 何欣[2] 周珉[2] 吴鸿[2] Zhang Shunrong;Li Dongfang;He Jiqin;Jiao Jiao;Li Yuming;He Xin;Zhou Min;Wu Hong(Guangxi International Zhuang Medicine Hospital,Nanning 530201,China;Department of Integrated Chinese and Western Medicine,Hunan Cancer Hospital,Changsha 410006,China;Department of Oncology,Changsha Eighth Hospital,Changsha 410100,China)

机构地区：[1]广西国际壮医医院,南宁530201 [2]湖南省肿瘤医院中西医结合科,长沙410006 [3]长沙市第八医院肿瘤科,长沙410100

出　　处：《世界中医药》2019年第10期2618-2622,共5页World Chinese Medicine

基　　金：湖南省自然科学基金面上项目(2017JJ2174);湖南省中医药管理局重点项目(201719)

摘　　要：目的:观察胃复方对人胃癌BGC-823裸鼠的抑瘤作用及c-Myc、人端粒酶逆转录酶(hTERT)基因表达的影响,评估胃复方作用及其可能的作用机制。方法:建立人胃癌BGC-823裸鼠模型;将成功建立的裸鼠胃癌皮下移植瘤模型的40只荷瘤鼠随机分为5组(n=8):模型对照组、胃复方低剂量组、胃复方高剂量组、氟尿嘧啶组、氟尿嘧啶联合中药组,连续给药4周。每7天记录肿瘤长径、短径,计算瘤体体积并绘制肿瘤生长曲线图;给药4周后处死所有荷瘤鼠,剥离皮下移植瘤,称瘤重、计算抑瘤率;免疫组织化学法检测瘤体组织c-Myc、hTERT蛋白表达。结果:1)胃复方对人胃癌BGC-823裸鼠移植瘤具有明显抑制作用,各剂量间有量效关系。其中胃复方低剂量组、胃复方高剂量组、氟尿嘧啶组、氟尿嘧啶联合中药组的抑瘤率分别为21.06%、45.89%、30.65%、59.42%。氟尿嘧啶联合中药组效果最好。2)瘤重显示与模型组比较,各药物组瘤重均有不同程度的减轻,差异有统计学意义(P<0.05);其中氟尿嘧啶联合中药组降低明显。3)肿瘤生长曲线图显示与模型组比较,各药物组瘤体积均有一定程度缩小,差异有统计学意义(P<0.05)。4)与模型组比较,药物组均能下调c-Myc、hTERT蛋白表达量,差异有统计学意义(P<0.05),其中氟尿嘧啶联合中药组下降最明显。结论:胃复方能够抑制人胃癌BGC-823细胞BALB/c-nu裸鼠皮下移植瘤生长,可能与下调c-Myc、hTERT蛋白表达有关,并且与氟尿嘧啶联合用药有增效作用。Objective:To observe tumor inhibiting effect of Weifu Formula in nude mice with human gastric cancer BGC-823 and its effects on gene expression of C-Myc and hTERT,and to evaluate the effects of Weifu Formula and its possible mechanism of action.Methods:The nude mouse model with human gastric cancer BGC-823 was established.A total of 40 tumor-bearing mice were performed successful modeling for nude mice with subcutaneous xenograft tumor of gastric cancer.They were randomly divided into 5 groups(n=8):model group,low dose Weifu Formula group,high dose Weifu Formula group,5-Fu group,and 5-Fu combined with Chinese materia medica group,with continuous administration for 4 weeks.Long diameter and short diameter of tumor were recorded every 7 d,tumor volume was calculated and tumor growth curve chart was drawn.All the tumor-bearing mice were killed after 4 weeks of administration,and the subcutaneous xenograft tumor was resected.The tumor was weighed and tumor inhibition rate was calculated.Protein expression of C-Myc and hTERT in tumor tissue was detected by immunohistochemistry.Results:1)Weifu Formula had obvious inhibiting effect on xenograft tumor in nude mice with human gastric cancer BGC-823 and there was dose-effect relationship between different doses.The tumor inhibition rates in the low dose Weifu Formula group,the high dose Weifu Formula group,the 5-Fu group and the 5-Fu combined with Chinese materia medica group were 21.06%,45.89%,30.65%and 59.42%,respectively.Efficacy in the 5-Fu combined with Chinese materia medica group was the best.2)In terms of tumor weight compared with the model group,the tumor weight in each medicine group showed different degrees of decrease(P<0.05).Among them,the decrease in the 5-Fu combined with Chinese materia medica group was significant.3)The tumor growth curve chart showed that the tumor volume in each medicine group was decreased to some extent compared with the model group(P<0.05).4)In comparison with the model group,the immunohistochemical results showed that the expression

关 键 词：胃复方 胃癌BGC-823细胞 移植瘤 C-MYC基因 人端粒酶逆转录酶基因 

分 类 号：R242[医药卫生—中医临床基础] R735.2[医药卫生—中医学]