HPLC-DAD法同时测定冠心康胶囊中4种活性成分的含量  

Simultaneous determination of four active components in Guanxinkang Capsules by HPLC-DAD

作　　者：黄洁文 陈燕霞[1] 谭汉添[1] HUANG Jie-wen;CHEN Yan-xia;TAN Han-tian(Department of Pharmacy,NO.1 Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一附属医院药学部

出　　处：《实用药物与临床》2019年第11期1186-1189,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立高效液相色谱法同时测定冠心康胶囊中芍药苷、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA含量的方法。方法色谱柱:Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相:乙腈-0.1%磷酸梯度洗脱;流速1.0 ml/min;柱温30℃,检测波长230 nm和270 nm,进样量:10μl。结果冠心康胶囊中芍药苷在23.50~2350.39 ng(r=0.9999)、隐丹参酮在4.07~407.20 ng(r=1.0000)、丹参酮Ⅰ在5.54~554.20 ng(r=1.0000)、丹参酮ⅡA在4.07~407.47 ng(r=0.9999)范围内线性良好,平均回收率分别为95.5%(RSD为0.5%)、97.1%(RSD为2.4%)、98.8%(RSD为1.7%)、96.6%(RSD为0.5%)。结论该方法经方法学验,证具有操作简单、快速、准确的优点,可以用于冠心康胶囊质量的全面控制。Objective To establish an HPLC-DAD method for simultaneous determination of peoniflorin,cryptotanshinone,tanshinoneⅠand tanshinoneⅡA in Guanxinkang Capsules.Methods Agilent TC-C18(4.6 mm×250 mm,5μm)column was used with acetonitrile-0.1%phosphoric acid as the mobile phase by gradient elution,at flow rate of 1.0 ml/min;column temperature was maintained at 30℃,detection wavelength was set at 230 nm and 270 nm,and the injection volume was 10μl.Results The linear ranges were 23.50~2350.39 ng(r=0.9999)for peoniflorin,4.07~407.20 ng(r=1.0000)for cryptotanshinone,5.54~554.20 ng(r=1.0000)for tanshinone I and 4.07~407.47μg(r=0.9999)for tanshinoneⅡA.The average recoveries of peoniflorin,cryptotanshinone,tanshinoneⅠand tanshinoneⅡA were 95.5%(RSD=0.5%),97.1%(RSD=2.4%),98.8%(RSD=1.7%)and 96.6%(RSD=0.5%),respectively.Conclusion The proposed method has advantages of simple operation,rapid analysis and high accuracy which is proved by methodology validation.This method is suitable for the quality control of Guanxinkang Capsule.

关 键 词：冠心康胶囊 芍药苷 隐丹参酮 丹参酮Ⅰ 丹参酮ⅡA 含量测定 

分 类 号：R28[医药卫生—中药学]