盐度渐变对黄条鰤消化酶和超氧化物歧化酶活力及甲状腺激素的影响  

Effect of Gradual Salinity Change on Digestive Enzyme,Superoxide Dismutase Activities and Thyroid Hormone Content of Yellowtail Kingfish(Seriola aureovittata)

作　　者：史宝[1] 柳学周[1,2] 刘永山[1,2] 张言祥 高全义 徐永江[1] 王滨[1] 姜燕[1] 宋雪松[1] SHI Bao;LIU Xue-Zhou;LIU Yong-Shan;ZHANG Yan-Xiang;GAO Quan-Yi;XU Yong-Jiang;WANG Bin;JIANG Yan;SONG Xue-Song(Laboratory for Marine Fisheries Science and Food Production Processes,Pilot Qingdao National Laboratory for Marine Science and Technology(Qingdao),Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Dalian Fugu Fishery Co.,Ltd.,Dalian 116400,China)

机构地区：[1]青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室,中国水产科学研究院黄海水产研究所,山东青岛266071 [2]上海海洋大学水产与生命学院,上海201306 [3]大连富谷水产有限公司,辽宁大连116400

出　　处：《中国海洋大学学报（自然科学版）》2020年第1期48-56,共9页Periodical of Ocean University of China

基　　金：中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金项目(2018GH17);中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022017016);青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室开放课题项目(2017-3A01);青岛市民生科技计划项目(17-3-3-61-nsh);国家自然科学基金项目(31772829);国家海水鱼技术体系项目(CARS-47)资助~~

摘　　要：为探讨黄条鰤(Seriola aureovittata)对盐度渐变的应激响应机制,设置盐度5、10、15、20、29和35共6个盐度渐变点,对幼鱼的消化酶活力、超氧化物歧化酶(SOD)和甲状腺激素(T4)等生理指标进行了检测和分析。研究显示:胃、肠、肝脏和幽门盲囊的脂肪酶的活性均在盐度29渐变点最高,在低盐度渐变点这4个组织的脂肪酶活性均随盐度降低而降低。肠和肝脏的蛋白酶活性在盐度29渐变点达到峰值,幽门盲囊的蛋白酶活性则在盐度35渐变点最高,且与其他盐度渐变点间有显著性差异(P<0.05)。胃、肠、肝脏的淀粉酶活性在盐度29渐变点最高,而幽门盲囊的淀粉酶活性在盐度35渐变点最高,但与盐度29渐变点无显著性差异(P>0.05)。血清SOD活性在盐度5渐变点显著低于其他盐度渐变点(P<0.05),SOD活性在盐度20和35渐变点与29渐变点无显著性差异(P>0.05)。血清T4浓度随着盐度的升高或降低均升高,盐度20渐变点与29渐变点的T4浓度无显著性差异(P>0.05)。研究显示,自然海水盐度29是黄条鰤幼鱼存活的适宜盐度,且黄条鰤幼鱼能较快适应略低盐度(20~29),但较低或较高盐度渐变点的消化酶活力和抗应激指标则显著异常。盐度渐变可引起黄条鰤幼鱼的消化生理、抗氧化水平和T4浓度的变化,黄条鰤幼鱼对外界盐度变化表现出较强的适应性。In order to understand the influence of the gradual salinity change on the stress physiological response of juvenile Seriola aureovittata, the 5, 10, 15, 20, 29 and 35 were set a salinity gradient. The activities of the digestive enzymes and superoxide dismutase(SOD) and content of thyroid hormone(T4) were analyzed. The results showed that the lipase activity of the stomach, intestine, liver and pyloric cystic was the highest at 29, the lipase activity of the four tissues at low salinities decreased with salinity. The activity of protease in the intestine and liver peaked at 29, and its differences from other salinities were significant(P<0.05). The activity of protease in the pyloric cystic was the highest at 35, and was significantly different from that of other salinities(P<0.05). The amylase activity in the stomach, intestinal and liver peaked at 29. The amylase activity of the pyloric cystic was the highest at 35, but not significantly different from that at 29(P>0.05). The activity of serum SOD at salinity 5 was significantly lower than that at other salinities(P<0.05). The activity of SOD was no significant different among salinities 20, 35 and 29(P>0.05). The concentration of serum T4 increased with the decrease of salinity. The concentration of serum T4 was not significantly different between 20 and 29(P>0.05). These results indicated that the natural seawater(salinity 29) is optimal to the survival of juvenile S. aureovittata. This species can adapt quickly to slightly low salinities between 20 and 29. However, the digestive enzyme activity and anti-stress index at other salinities were not normal. The juvenile S. aureovittata has a strong adaptability to external salinity change within the salinity range.

关 键 词：黄条鰤 盐度渐变 消化酶 超氧化物歧化酶 甲状腺激素 应激响应 

分 类 号：S96[农业科学—水产养殖]