天山堇菜总黄酮抗菌、抗炎、解热、镇咳作用研究  

Study on the antibacterial, anti-inflammatory, antipyretic and antitussive effects of total flavonoids of Viola thianschanica Maxim.

作　　者：王雪 史玉柱 司丽君 赵丽娟 马雪萍 黄华 WANG Xue;SHI Yuzhu;SI Lijun;ZHAO Lijuan;MA Xueping;HUANG Hua(Xinjiang Institute of Materia Medica,Xinjiang Uygur Autonomous Region,Urumqi 830004,China)

机构地区：[1]新疆维吾尔自治区药物研究所

出　　处：《中国医药导报》2019年第29期21-24,共4页China Medical Herald

基　　金：新疆维吾尔自治区重点研发计划项目（2016B03044-5）

摘　　要：目的考察天山堇菜总黄酮的抗菌、抗炎、解热、镇咳作用。方法采用体外倍比稀释法及体内建立小鼠金黄色葡萄球菌、大肠埃希菌感染模型来考察天山堇菜总黄酮的体内外抗菌作用;采用小鼠耳廓肿胀模型和腹腔毛细血管通透性升高模型考察其抗炎作用;通过干酵母诱导的大鼠发热模型考察其解热作用;采用氨水引咳法观察其镇咳作用。结果天山堇菜总黄酮对金黄色葡萄球菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)值分别为1.95、3.90 mg/mL,对大肠埃希菌的MIC、MBC值分别为0.49、0.98 mg/mL。天山堇菜总黄酮在0.32 g/kg对金黄色葡萄球菌、大肠埃希菌小鼠感染模型具有显著的保护作用,其保护率分别为30%、40%。天山堇菜总黄酮在0.16、0.32 g/kg可显著抑制二甲苯致小鼠耳廓肿胀,在0.08、0.16、0.32 g/kg可显著抑制冰醋酸致小鼠腹腔毛细血管炎性渗出;天山堇菜总黄酮在0.14、0.28 g/kg对干酵母致大鼠发热具有较好的抑制作用。天山堇菜总黄酮对氨水引咳具有显著的抑制作用,在0.32 g/kg时抑制率为57.7%。结论天山堇菜总黄酮提取物具有较好的抗菌、抗炎、解热及镇咳药理活性,其作用机制有待于进一步研究。Objective To investigate the antibacterial,anti-inflammatory,antipyretic and antitussive effects of total flavonoids of Viola thianschanica Maxim..Methods The doubling dilution method in vitro and establishment of mice infection model of Staphylococcus aureus,Escherichia coli in vivo were used to investigate the antibacterial effect of total flavonoids of Viola thianschanica Maxim.in vivo and in vitro.The model mice with auricle swelling and model mice with increased peritoneal capillary permeability were used to investigate the anti-inflammatory effects.The model rats with fever induced by dry yeast were adopted to investigate the antipyretic effect.The model rats with cough induced by ammonia water were adopted to investigate the antitussive effect.Results The minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)values of total flavonoids of Viola thianschanica Maxim.against Staphylococcus aureus were 1.95,3.90 mg/mL respectively,and the MIC and MBC values of total flavonoids of Viola thianschanica Maxim.against Escherichia coli were 0.49,0.98 mg/mL respectively.The total flavonoids of Viola thianschanica Maxim.had a significant protective effect on the infection model of Staphylococcus aureus and Escherichia coli in mice at 0.32 g/kg,the protective rates were 30% and 40%respectively.The total flavonoids of Viola thianschanica Maxim.could significantly inhibit auricle swelling induced by xylene in mice at 0.16,0.32 g/kg,and could significantly inhibit capillary inflammatory exudation induced by glacial acetic acid in mice at 0.08,0.16,0.32 g/kg.The total flavonoids of Viola thianschanica Maxim.had a good inhibitory effect on fever induced by dry yeast in rats at 0.14,0.28 g/kg.The total flavonoids of Viola thianschanica Maxim.had a significant inhibitory effect on cough induced by ammonia water,the inhibitory rate was 57.7% at 0.32 g/kg.Conclusion The total flavonoids extract of Viola thianschanica Maxim.has good antibacterial,anti-inflammatory,antipyretic and antitussive pharmacologi

关 键 词：天山堇菜总黄酮 抗菌 抗炎 解热 镇咳 

分 类 号：R285.5[医药卫生—中药学]