反相HPLC测定精制马拉硫磷溶液方法探讨  

Investigation determination of content of purified malathion solution

作　　者：陈向丹[1] 王峻[1] 胡深[1] 万珊[1] 董书香 CHEN Xiang-dan;WANG Jun;HU Shen;WAN Shan;DONG Shu-xiang(Hubei Province Institute of Veterinary Drug,Wuhan 430070,China)

机构地区：[1]湖北省兽药监察所

出　　处：《湖北农业科学》2019年第21期170-172,共3页Hubei Agricultural Sciences

摘　　要：建立了精制马拉硫磷溶液中马拉硫磷含量的反相HPLC测定方法。采用C18色谱柱(4.6 mm×150 mm,5μm),检测波长225 nm,流动相为甲醇∶水(60∶40),流速1.0 mL/min。结果表明,马拉硫磷在0.050~1.500 mg/mL与峰面积呈良好的线性关系,相关系数为0.9998,平均回收率为101.6%,RSD为0.9%。该方法简便、准确、快速、重现性好,可用于该制剂的含量测定。To establish a method for the determination of malathion content in malathion solution by RP-HPLC.The C18 chromatographic column(4.6 mm×150 mm,5μm)was used to detect the wavelength 225 nm,the mobile phase was methanol water(60∶40),and the flow rate was 1.0 mL/min.The results showed that malathion in the range of 0.050~1.500 mg/mL,there was a good linear relationship between concentration and peak area,the correlation coefficient was 0.9998(n=6),the average recovery rate was 101.6%,RSD was 0.9%;the method is simple,accurate,rapid and reproducible,and can be used for determination of the content of this preparation.

关 键 词：马拉硫磷 反相HPLC法 含量测定 

分 类 号：O657.72[理学—分析化学]