化学去细胞异体神经联合骨髓间充质干细胞修复损伤坐骨神经的生物力学评价  

Biomechanical analysis of chemical acellular nerve allograft combined with bone marrow mesenchymal stem cell transplantation for repairing sciatic nerve injury

作　　者：吴志峰 罗民[2] Wu Zhifeng;Luo Min(Department of Orthopedics,Jilin General Hospital,Chinese People’s Armed Police Force,Changchun 130041,Jilin Province,China;Department of Pain,China-Japan Friendship Hospital,Jilin University,Changchun 130031,Jilin Province,China)

机构地区：[1]武装警察部队吉林省总队医院骨科,吉林省长春市130041 [2]吉林大学中日联谊医院疼痛科,吉林省长春市130031

出　　处：《中国组织工程研究》2020年第7期991-995,共5页Chinese Journal of Tissue Engineering Research

基　　金：吉林省省级产业创新专项资金项目(2016c041-3)，项目负责人：罗民~~

摘　　要：背景:以拉伸力学指标评估化学去细胞异体神经联合骨髓间充质干细胞治疗坐骨神经损伤的效果,目前鲜有报道。目的:评估化学去细胞异体神经联合骨髓间充质干细胞移植治疗坐骨神经损伤的效果。方法:将45只SD大鼠随机分为自体神经移植组、化学去细胞异体神经移植组、化学去细胞异体神经联合骨髓间充质干细胞移植组,每组15只,制备10 mm坐骨神经损伤模型,分别以自体神经、化学去细胞异体神经、化学去细胞异体神经联合骨髓间充质干细胞修复坐骨神经损伤,术后20周,进行坐骨神经电生理检测和坐骨神经单向拉伸实验。结果与结论:①化学去细胞异体神经联合骨髓间充质干细胞移植组与自体神经移植组波幅值、运动传导速度值大于化学去细胞异体神经移植组(P<0.05);②化学去细胞异体神经联合骨髓间充质干细胞移植组与自体神经移植组拉伸弹性限度应变、弹性限度应力、最大应变、最大应力大于化学去细胞异体神经移植组(P<0.05);③结果表明,化学去细胞异体神经联合骨髓间充质干细胞移植对坐骨神经损伤具有明显的修复效果。BACKGROUND:It is seldom reported that the effect of chemical acellular nerve allograft combined with bone marrow mesenchymal stem cell transplantation on sciatic nerve injury is evaluated by tensile mechanical properties.OBJECTIVE:To determine the effect of chemical acellular nerve allograft combined with bone marrow mesenchymal stem cell transplantation in the treatment of sciatic nerve injury.METHODS:Forty-five Sprague-Dawley rats were randomly divided into autologous nerve transplantation group,chemical acellular nerve allograft group,and chemical acellular nerve allograft and bone marrow mesenchymal stem cell transplantation group(n=15 per group).Models of 10-mm sciatic nerve injury were prepared.The injured sciatic nerves were repaired using autologous nerve,chemical acellular nerve allograft and chemical acellular nerve allograft and bone marrow mesenchymal stem cells.At 20 weeks after surgery,electrophysiological measurement and stretching experiments were carried out.RESULTS AND CONCLUSION:(1)The wave amplitude and motion conduction velocity were larger in the chemical acellular nerve allograft and bone marrow mesenchymal stem cell transplantation group and autologous nerve transplantation group than in the chemical acellular nerve allograft group(P<0.05).(2)The tensile elastic limit strain,elastic limit stress,maximum strain and maximum stress were larger in the chemical acellular nerve allograft and bone marrow mesenchymal stem cell transplantation group and autologous nerve transplantation group than in the chemical acellular nerve allograft group(P<0.05).(3)These results suggest that chemical acellular nerve allograft and bone marrow mesenchymal stem cell transplantation has obvious effect on repairing sciatic nerve injury.

关 键 词：坐骨神经损伤 化学去细胞异体神经 骨髓间充质干细胞 神经修复 生物力学 

分 类 号：R459.9[医药卫生—治疗学] R318.01[医药卫生—临床医学]