黄芩素能下调诱捕受体3表达抑制肝癌干细胞的生物学行为  

Baicalein inhibits the biological behavior of hepatocellular carcinoma stem cells by downregulation of Decoy receptor 3 expression

作　　者：岑妍慧[1,2,3] 夏猛[1] 贾微[1] 罗伟生[1] 林江[1] 陈松林[2] 陈炜[3] 刘鹏[1] 黎明星 李景云[1] 李曼莉[1] 艾丁丁 蒋云霞[1] Cen Yanhui;Xia Meng;Jia Wei;Luo Weisheng;Lin Jiang;Chen Songlin;Chen Wei;Liu Peng;Li Mingxing;Li Jingyun;Li Manli;Ai Dingding;Jiang Yunxia(Guangxi University of Chinese Medicine,Nanning 530001,Guangxi Zhuang Autonomous Region,China;First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi Zhuang Autonomous Region,China;Guangxi Key Laboratory of Basic Research in Traditional Chinese Medicine,Guangxi University of Chinese Medicine,Nanning 530001,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西中医药大学,广西壮族自治区南宁市530001 [2]广西中医药大学第一附属医院,广西壮族自治区南宁市530023 [3]广西中医药大学广西中医基础研究重点实验室,广西壮族自治区南宁市530001

出　　处：《中国组织工程研究》2020年第7期1023-1029,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(8166150270)，项目负责人：罗伟生;中国博士后科学基金(2017M6232)，项目负责人：岑妍慧;广西特聘专家团队(GZKZ-G1103)，项目负责人：林江;广西壮族自治区卫生厅中医药科技专项课题(GZBZ16-07,GZLC16-23)，项目负责人：岑妍慧、贾微;广西中医药大学岐黄工程高层次人才团队培育项目(2018001)，项目负责人：夏猛;广西中医基础研究重点实验室开放课题(17-259-49-09)，项目负责人：岑妍慧;广西中医基础研究重点实验室开放课题，项目负责人：贾微;广西一流学科建设项目重点课题(2018XK023,2018XK024)，项目负责人：岑妍慧、贾微;广西一流学科建设开放课题(2019XK137)，项目负责人：岑妍慧;广西壮瑶药重点实验室建设项目(GXZYKF-201708)，项目负责人：贾微;广西高校中青年教师科研基础能力提升项目(2019KY0327)，项目负责人：刘鹏;2019年广西中医药大学校级科研项目(2019MS001)，项目负责人：贾微~~

摘　　要：背景:目前尚未见有关以肝癌干细胞为靶向的中草药抗肝癌机制的报道,因此有必要展开相关研究。目的:探讨黄芩素对肝癌干细胞诱捕受体3表达的影响,以及下调诱捕受体3表达对肝癌干细胞生物学行为的影响。方法:按照课题组之前建立的技术方法,从肝癌细胞株(购自中国科学院上海细胞库)中获得肝癌干细胞。将肝癌干细胞分为黄芩素高、中、低剂量组以及对照组,黄芩素高、中、低剂量组分别用含200,100,50μmol/L黄芩素的L-DMEM培养基培养,对照组仅用L-DMEM培养基培养。采用RT-PCR、Western blot检测黄芩素处理后诱捕受体3 mRNA和蛋白表达变化,采用CCK8法、流式细胞术、Transwell法检测肝癌干细胞增殖、细胞周期分布、凋亡和迁移情况。结果与结论:①与对照组相比,高剂量黄芩素能显著下调肝癌干细胞诱捕受体3的mRNA和蛋白表达,因此确认高剂量黄芩素为最佳作用浓度;②与对照组相比,高剂量黄芩素组肝癌干细胞的增殖能力明显下降,S期和G2期细胞比例增加,而G1期的细胞比例则减少(P<0.05);③与对照组相比,高剂量黄芩素组肝癌干细胞的凋亡率明显增加,迁移能力明显下降;④结果表明,高剂量黄芩素能通过下调肝癌干细胞的诱捕受体3表达而抑制细胞的一系列生物学行为,为临床上以诱捕受体3为靶点,以肝癌干细胞为对象进行肝癌治疗提供了实验依据。BACKGROUND:To date,there is no report on the anti-hepatoma mechanism of Chinese herbal medicine targeting hepatocellular carcinoma stem cells;therefore,relevant research is necessary.OBJECTIVE:To observe the effect of baicalein on the expression of Decoy receptor 3 in hepatocellular carcinoma stem cells as well as the biological behavior of hepatocellular carcinoma stem cells after down-regulation of Decoy receptor 3.METHODS:Hepatocellular carcinoma stem cells were obtained from hepatocellular carcinoma cell lines(purchased from the Cell Bank,Chinese Academy of Sciences,Shanghai,China),cultured and passaged.The cells were cultured in the low-glucose DMEM containing nothing(control group),200(high-dose group),100(middle-dose group)or 50μmol/L(low-dose group)baicalein.After treatment with baicalein,RT-PCR and western blot were used to detect the expression of decoy receptor 3 at mRNA and protein levels.Cell counting kit-8,flow cytometry,and Transwell assay were used to detect the proliferation,cell cycle distribution,apoptosis and migration of hepatocellular carcinoma stem cells.RESULTS AND CONCLUSION:Compared with the control group,high-dose baicalein could significantly down-regulated the expression of decoy receptor 3 mRNA and protein in hepatocellular carcinoma stem cells.High-dose baicalein was then confirmed to be the best concentration of action.Compared with the control group,in the high-dose baicalein group,the proliferation ability of hepatocellular carcinoma stem cells decreased significantly,the percentage of S-phase and G2-phase cells increased,while the percentage of G1-phase cells decreased(P<0.05).Compared with the control group,in the high-dose baicalein group,the apoptotic rate of hepatocellular carcinoma stem cells increased significantly,while the migration ability decreased markedly.To conclude,high-dose baicalein can inhibit a series of biological behaviors of hepatocellular carcinoma stem cells by down-regulating the expression of Decoy receptor 3,which provides an experimental basis for cli

关 键 词：黄芩素 肝癌干细胞 诱捕受体3 细胞增殖 细胞周期 细胞凋亡 细胞迁移 国家自然科学基金 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]