不同氧化剂辅助光催化反应对提高木质纤维素酶解效果的影响  

Effect of Oxidants-Assisted Photocatalytic Pretreatment on Enhancing Enzymatic Hydrolysis of Lignocellulose

作　　者：王小琴 张耿崚 黄志华 杨佘维 韩业钜 王玉洁 刘敬勇 WANG Xiaoqin;ZHANG Gengling;HUANG Zhihua;YANG Shewei;HAN Yeju;WANG Yujie;LIU Jingyong(Guangdong Provincial Academy of Environment Science,Guangzhou 510045,China;Institute of Environmental Health and Pollution Control,School of Environmental Science and Engineering,Guangdong University of Technology,Guangzhou 510006,China;South China Institute of Environmental Science.MEE,Guangzhou 510530,China)

机构地区：[1]广东省环境科学研究院,广东广州510045 [2]广东工业大学环境科学与工程学院,环境健康与污染控制研究院,广东广州510006 [3]生态环境部华南环境科学研究所,广东广州510530

出　　处：《环境科学研究》2019年第11期1921-1927,共7页Research of Environmental Sciences

基　　金：广东省科技计划项目(No.2017B040404007);广东省公益研究与能力建设专项(No.2016A020221019);广东省自然科学基金(No.2017A030310323)~~

摘　　要：木质纤维素以其可再生、丰富等优点成为可替代能源的理想选择,然而木质纤维素的致密结构使得后续的酶解过程极其困难,因此木质纤维素的预处理显得尤为重要.为了探讨氧化剂对光催化反应预处理木质纤维素的影响,在TiO2/UV体系中添加不同种类的氧化剂对稻秆进行预处理,考察TiO2/UV体系中影响光催化的因素〔ρ(TiO2)、溶液pH及光催化时间〕,并进一步探讨在TiO2/UV体系中添加O3、SPC(过碳酸钠)、K2S2O8及KIO4等氧化剂对稻秆酶解效果的影响.结果表明:在TiO2 UV体系中,当光催化时间为3 h、ρ(TiO2)为10 g/L、pH为8时,稻秆的酶解效果最佳(310.17 mg/g).TiO2/UV/O3光催化体系对稻秆的预处理效果强于TiO2/UV氧化剂体系,TiO2/UV/O3体系预处理稻秆酶解后产生的w(还原糖)最高,达到356.40 mg/g.TiO2/UV/O3体系预处理稻秆成分分析显示,木质素去除率为15.52%.FE-SEM(场发射扫描电镜)、FT-IR(红外光谱)及PXRD(X-射线粉末衍射)等表征分析结果表明,TiO2/UV/O3体系预处理能够对稻秆结构进行破坏.研究显示,TiO2/UV/O3光催化体系对稻秆进行预处理能够有效提高酶解效率.Lignocellulose is an ideal alternative energy source because of its renewable and abundant properties.However,the recalcitrant structure of lignocellulose makes the subsequent enzymatic hydrolysis process extremely difficult.Therefore,the pretreatment of lignocellulose is particularly important.To evaluate the effect of oxidants on the photocatalysis pretreatment of lignocellulose,different oxidants were added to the TiO2/UV system to pretreat the rice straw.The effects of TiO2 concentrations,pH and photocatalysis time were investigated.Furthermore,the effects of oxidants such as O3,SPC,K2S2O8 and KIO4 on the enzymatic hydrolysis of rice straw in TiO2/ UV system were discussed.The results indicated that the optimal conditions for TiO2 /UV pretreatment of rice straw were pH=8 for 3 h with 10 mg L,resulting in a maximum released reducing sugar concentration of 310.17 mg/ g.The released reducing sugar from the enzymatic hydrolysis of the TiO2/UV/O3-pretrated rice straw was higher than that from the rice straw pretreated with TiO2/ UV /SPC,TiO2/UV /K2S2O8 and TiO2 /UV KIO4.Moreover,the maximum released reducing sugar concentration was 356.40 mg/ g of TiO2/UV/O3-pretreated rice straw.Composition analyses of rice straw after the TiO2/UV/O3 pretreatment showed that the removal rate of the lignin content was 15.52%.The effects of TiO2/UV/O3 pretreatment were characterized by FE-SEM,FT-IR and PXRD,which indicated that the structures of rice straw could be destroyed.This work confirmed that the pretreatment of rice straw with TiO2/UV/O3 is an effective method.

关 键 词：光催化 氧化剂 臭氧(O3) 预处理 酶解 木质纤维素 

分 类 号：X705[环境科学与工程—环境工程]