迷迭香中2个新松香烷型二萜化合物  

Two new abietane diterpenes from Rosmarinus officinalis

作　　者：袁瑞瑛[1,2] 卓玛东智 韦玉璐[2] 黄蓉 余建清 蔡由生[2] YUAN Rui-ying;ZHUOMA-Dongzhi;WEI Yu-lu;HUANG Rong;YU Jian-qing;CAI You-sheng(Medical College,Tibet University,Lhasa 850012,China;School of Pharmaceutical Sciences,Wuhan University,Wuhan 430071,China)

机构地区：[1]西藏大学医学院,西藏拉萨850012 [2]武汉大学药学院,湖北武汉430071

出　　处：《中草药》2019年第20期4853-4858,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的研究迷迭香Rosmarinus officinalis的化学成分及其活性。方法采用多种柱色谱对迷迭香地上部分甲醇提取物进行分离纯化;通过波谱数据对化合物进行结构鉴定;采用磺酰罗丹明B比色法(SRB法)对所得化合物进行细胞毒活性测试。结果从迷迭香的甲醇提取物中分离得到2个新的松香烷型二萜类化合物和8个已知二萜类化合物,分别鉴定为7-异丙氧基-表异迷迭香酚(1)、7-异丙氧基-迷迭香醌(2)、迷迭香酚(3)、7-甲氧基-迷迭香酚(4)、表迷迭香酚(5)、7-甲氧基-表异迷迭香酚(6)、rosmaquinoneB(7)、鼠尾草酚(8)、galdosol(9)和pisiferal(10)。结论化合物1和2为新化合物,分别命名为7-异丙氧基-表异迷迭香酚和7-异丙氧基-迷迭香醌。活性测试结果表明所有二萜类化合物均无明显细胞毒活性。Objective To study the chemical constituents of aerial part of Rosmarinus officinalis.Methods Compounds were isolated and purified by repeated column chromatography on silica gel column and Sephadex LH-20 gel column.The structures of the new compounds were elucidated by analysis of their NMR and HRESIMS data.The cytotoxicity of the compounds was evaluated by SRB methods.Results Ten compounds were obtained and identified as 7-O-isopropyl-epirosmanol(1),7-O-isopropyl rosmaquinone(2),rosmanol(3),7-O-methyl-rosmanol(4),epirosmanol(5),7-methoxy-epirosmanol(6),rosmaquinone B(7),carnosol(8),galdosol(9)and pisiferal(10).Conclusion Compounds 1 and 2 are new abietane diterpenes named 7-O-isopropyl-epirosmanol(1),7-O-isopropyl rosmaquinone(2),and showed no cytotoxicity.

关 键 词：迷迭香 松香烷型二萜 7-异丙氧基-表异迷迭香酚 7-异丙氧基-迷迭香醌 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]