黔产白及中糖苷类化学成分研究  

Glycoside constituents from Bletilla striata

作　　者：陈长芬 蒋赛 娄华勇[1,2] 万科 马小攀[2] 吴明开 夏与 张妮[1,2] 潘卫东 CHEN Chang-fen;JIANG Sai;LOU Hua-yong;WAN Ke;MA Xiao-pan;WU Ming-kai;XIA Yu;ZHANG Ni;PAN Wei-dong(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;Key Laboratory of Natural Product Chemistry of Guizhou Academy of Sciences,Guiyang 550014,China;School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Institute of Modern Chinese Medicinal Materials of Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Anlong Xinman Biotechnology Co.,Ltd.,Anlong 552400,China)

机构地区：[1]贵州医科大学,省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [2]贵州省中国科学院天然产物化学重点实验室,创新药物研究中心,贵州贵阳550014 [3]湖南中医药大学药学院,湖南长沙410208 [4]贵州省农业科学院贵州省现代中药材研究所,贵州贵阳550006 [5]安龙县欣蔓生物科技有限责任公司,贵州安龙552400

出　　处：《中草药》2019年第20期4879-4883,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(U1812403);国家自然科学基金资助项目(81660580);贵州省科技计划项目(QKHZC [2019] 2753,QKHPTRC [2017] 5737,QKHPTRC [2016] 2832);贵州省高层次创新人才培养计划项目(QKHRC [2016] 4037);中央引导地方科技发展专项(QKHZYD [2017] 4001);贵州省科技创新人才团队建设项目(QKHRCTD [2015] 4026);贵州省中药材产业体系建设项目(GZCYTX2015-0202)

摘　　要：目的研究白及Bletilla striata中的糖苷类化学成分。方法采用硅胶柱色谱、Sephadex LH-20和半制备液相色谱等现代分离方法和技术对其化学成分进行分离纯化,并根据理化性质和波谱数据进行结构鉴定。结果从黔产白及95%乙醇提取物中分离得到了13个糖苷类化合物,分别鉴定为1-methyl-3-phenylpropyl-β-D-glucopyranoside(1)、1-(4-β-Dglucopyranosyl oxybenzyl) 4-ethyl (2R)-2-isobutylmalate(2)、3′,5-dimethoxy-bibenzyl-3-O-β-D-glucopyranoside(3)、syringaresinol mono-β-D-glucoside (4)、 4-O-(6′-O-glucosyl-p-coumaroyl)-4-hydroxybenzylalcohol (5)、(4-hydroxyphenyl)methyl-β-Dglucopyranoside(6)、4-methylphenyl-β-D-glucopyranoside(7)、benzyl-β-D-glucopyranoside(8)、phenyl-β-D-glucopyranoside(9)、4-[(acetyloxy) methyl] phenyl-β-D-glucopyranoside(10)、batatasin III-3-O-glucoside(11)、天麻素(12)、4-(4-β-D-glucopyranosyloxybenzyl)-(2R)-2-isobutylmalate(13)。结论化合物1是新天然产物,化合物3~6是首次从该植物中分离得到,化合物2、7~13为首次从白及属植物中分离得到。Objective To study the glycoside chemical constituents of Bletilla striata grown in Guizhou Province. Methods The glycoside chemical constituents were separated and purified by silica gel column chromatography, MCI, gel column chromatography, medium pressure preparation, highperformance liquid chromatography and other modern separation methods. The structures were identified based on the spectral data. Results Thirteen glycosides were isolated from the 95% ethanol extracts: 1-methyl-3-phenylpropyl-β-D-glucopyranoside(1), 1-(4-β-D-glucopyranosyl oxybenzyl) 4-ethyl(2 R)-2-isobutylmalate(2), 3′,5-dimethoxy-bibenzyl-3-O-β-D-glucopyranoside(3), syringaresinol mono-β-D-glucoside(4), 4-O-(6′-O-glucosyl-p-coumaroyl)-4-hydroxybenzyl alcohol(5),(4-hydroxyphenyl) methyl-β-D-glucopyranoside(6), 4-methylphenyl-β-D-glucopyranoside(7), benzyl-β-Dglucopyranoside(8), phenyl-β-D-glucopyranoside(9), 4-[(acetyloxy) methyl] phenyl-β-D-glucopyranoside(10), batatasin III-3-Oglucoside(11), gastrodin(12) and 4-(4-β-D-glucopyranosyl-oxybenzyl)-(2 R)-2-isobutylmalate(13). Conclusion Compound 1 is a new natural product. Compounds 3-6 are isolated from this plant for the first time;Compounds 2, 7-13 are obtained from this genus for the first time.

关 键 词：白及 糖苷类 1-methyl-3-phenylpropyl-β-D-glucopyranoside 4-methylphenyl-β-D-glucopyranoside 天麻素 

分 类 号：R284.1[医药卫生—中药学]