络合萃取异甘草苷的工艺条件研究  

Study on technological conditions of complex extraction of isoliquiritin

作　　者：孙晓燕[1] 王继龙[1] 刘晓霞[1] 石盘棋 周博 万玲娟 李真真 魏舒畅[1] SUN Xiao-yan;WANG Ji-long;LIU Xiao-xia;SHI Pan-qi;ZHOU Bo;WAN Ling-juan;LI Zhen-zhen;WEI Shu-chang(Research Center of Traditional Chinese Medicine Pharmaceutical Technology and Engineering of Gansu Province,Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学甘肃省中药制药工艺工程研究中心

出　　处：《中草药》2019年第20期4920-4924,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81460608);甘肃省基础研究创新群体项目(1506RJIA034)

摘　　要：目的探究络合萃取异甘草苷时萃取与反萃取工艺条件对异甘草苷转移情况的影响。方法以异甘草苷萃取率为指标,确定最佳的络合萃取剂组成及质量分数;以异甘草苷反萃取率为指标,考察反萃取剂的种类、质量分数,最终得到从甘草超滤液中萃取与反萃取制备异甘草苷的工艺条件。结果最佳络合萃取条件为三烷基氧化膦(TRPO)-磺化煤油(7∶93)对异甘草苷的萃取率达到97.60%。最佳反萃取剂为0.26%NaOH水溶液,异甘草苷的反萃取率达到95.40%。结论在该实验所得萃取与反萃取最佳条件下,可实现将异甘草苷从甘草超滤液转移至络合萃取剂再到碱性反萃取剂中,最终通过萃取反萃取得到异甘草苷。Objective To investigate the effect of extraction and reverse extraction conditions on the transfer of isoliquiritin. Methods The extraction rate of isoliquiritin was used as the index to determine the best composition and concentration of complexing extractant. Taking the reverse extraction rate of isoliquiritin as the index, the species and concentration of the reverse extraction agent were investigated, and finally the technological conditions for the extraction and reverse extraction of isoliquiritin from glycyrrhizin ultrafiltration were obtained. Results The best complexation extraction condition was: the ratio of TRPO to sulfonated kerosene was 7:93, and the extraction rate of isoliquiritin reached 97.60%. The best reverse extraction agent was 0.26% NaOH aqueous solution, and the reverse extraction rate of isoliquiritin reached 95.40%. Conclusion Under the optimal conditions of extraction and reverse extraction obtained in this experiment, isoliquiritin can be transferred from glycyrrhizin ultrafiltration to complexing extractant and then to alkaline reverse extraction agent, and finally isoliquiritin can be obtained by extraction and reverse extraction.

关 键 词：异甘草苷 甘草超滤液 三烷基氧化膦 磺化煤油 络合萃取 

分 类 号：R284.2[医药卫生—中药学]