杠柳毒苷对慢性心力衰竭大鼠的影响研究  

Effect of periplocoside on chronic heart failure rats

作　　者：林肖彬 黄宝英 卓冠航 LIN Xiao-bin;HUANG Bao-ying;ZHUO Guan-hang(Department of Cardiovascular Internal Medicine,Ningde Hospital,Fujian Medical University,Ningde 352100,Fujian Province,China;Department of Central Laboratory,Ningde Hospital,Fujian Medical University,Ningde 352100,Fujian Province,China;Department of Ultrasound Medicine,Ningde Hospital,Fujian Medical University,Ningde 352100,Fujian Province,China)

机构地区：[1]福建医科大学附属宁德市医院心血管内科,福建宁德352100 [2]福建医科大学附属宁德市医院中心实验室,福建宁德352100 [3]福建医科大学附属宁德市医院超声医学科,福建宁德352100

出　　处：《中国临床药理学杂志》2019年第21期2672-2674,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究杠柳毒苷对慢性心力衰竭大鼠的干预效果并分析其作用机制。方法SD大鼠采用结扎左冠状动脉前降支构建慢性心力衰竭大鼠模型,将建模成功的40只慢性心力衰竭大鼠随机分为模型组(n=20)及实验组(n=20),另选取20只大鼠仅穿线不结扎作为假手术组。实验组灌服10 mg·kg^-1杠柳毒苷,模型组及假手术组灌服等量生理盐水,每天1次,连续干预4周。用超声心动图检测大鼠左心室形态学指标及左心收缩功能指标,用苏木精-伊红(HE)染色观察心肌组织病理学形态,用逆转录-聚合酶链式反应(RT-PCR)及蛋白质印迹(WB)法检测心肌组织血管紧张素Ⅱ受体1(AT1)蛋白表达。结果假手术组、模型组及实验组大鼠左心室射血分数(LVEF)分别为(79.98±10.32)%,(51.32±9.85)%,(66.13±10.03)%;左心室短轴缩短率(FS)分别为(55.69±12.04)%,(21.07±8.33)%,(37.86±11.61)%,大鼠心肌组织AT1mRNA相对表达量分别为0.21±0.09,0.79±0.13,0.33±0.10,AT1蛋白相对表达量分别为0.12±0.01,0.85±0.04,0.19±0.03,差异均有统计学意义(均P<0.05)。模型组大鼠左心室舒张/收缩末期内径(LVDd/LVDs)较假手术组升高,而实验组较模型组降低(P<0.05)。结论杠柳毒苷可有效抑制慢性心力衰竭大鼠左心室重构,提高其左心室收缩功能,改善心肌病理学损伤,其作用机制与显著抑制心肌组织AT1蛋白表达相关。Objective To explore the effect on periplocoside of chronic heart failure rats and analyse the mechanism.Methods Chronic heart failure rat were built by ligation of left anterior descending coronary artery,the 40 cases chronic heart failure SD rats with success modeling were divided randomly into model group(n=20)and test group(n=20),another 20 cases rats were only thread without ligation and acted as sham-operation group.Test group were filling and uniformed 10 mg·kg-1 periplocoside,model group and sham-operation group were filling and uniformed the same amount saline,1 time·d-1,continuously intervention for 4 weeks.The left ventricular morphology indexes and left heart systolic function indexes were detected by echocardiographic;the myocardial tissue pathology morphology was observed by Hematoxylin and eosin(HE)staining;the myocardial tissue angiotensinⅡreceptor 1(AT1)expression was detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot(WB).Results The left ventricular ejection fraction(LVEF)in sham-operation group,model group and test group were(79.98±10.32)%,(51.32±9.85)%,(66.13±10.03)%,fraction shortening(FS)were(55.69±12.04)%,(21.07±8.33)%,(37.86±11.61)%,myocardial tissue AT1 mRNA relative expression were 0.21±0.09,0.79±0.13,0.33±0.10,the AT1 protein relative expression were 0.12±0.01,0.85±0.04,0.19±0.03,all with significant difference(all P<0.05).The left ventriular diastolic diameter/left ventriular systolic diameter(LVDd/LVDs)of rats in model group were higher than those in sham-operation group,while test group was lower than model group(P<0.05).Conclusion Periplocoside can inhibit the ventricular remodeling of chronic heart failure rat effectively,enhance the left ventricular systolic function,improve the myocardial pathological damage,the action mechanism related to the significant inhibition of myocardial tissue AT1 expression.

关 键 词：慢性心力衰竭 杠柳毒苷 超声心动图 血管紧张素Ⅱ受体1 表达 

分 类 号：R28[医药卫生—中药学]