辣椒碱对小鼠Lewis肺癌细胞LL/2的抗肿瘤作用研究  

Antitumor effects of capsaicin on cancerous cell LL/2 Lewis lung carcinoma

作　　者：高爱琴 柏春玲[2] 李秀英 GAO Ai-qin;BAI Chun-ling;LI Xiu-ying(Medical Faculty,Kunming University of Science and Technology,Kunming 650011,Yunnan Province,China;Experiment Center for Medical Science Research,Kunming Medical University,Kunming 650093,Yunnan Province,China)

机构地区：[1]昆明理工大学医学院,云南昆明650011 [2]昆明医科大学医学科学研究实验中心,云南昆明650093

出　　处：《中国临床药理学杂志》2019年第21期2679-2681,2686,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81560491)

摘　　要：目的研究辣椒碱对小鼠Lewis肺癌细胞LL/2的增殖、迁移和凋亡的影响及可能机制。方法将小鼠Lewis肺癌细胞LL/2分为2组:对照组和实验组。实验组用不同浓度(25,50,100,150,200,250μmol·L^-1)的辣椒碱处理48h,对照组细胞用相应浓度的DMSO(dimethyl sulfoxide)处理。用噻唑蓝实验检测细胞活力和半数抑制浓度(IC50);划痕实验检测100μmol·L^-1辣椒碱处理24 h后细胞迁移变化;克隆形成实验检测100μmol·L^-1辣椒碱处理10 d后细胞克隆形成的变化;Annexin V-FITC/PI和Hoechst 33258染色检测125μmol·L^-1辣椒碱处理24 h后细胞的凋亡;蛋白免疫印迹实验检测125μmol·L^-1辣椒碱处理24 h后细胞质中细胞色素C、活化型半胱氨酸天冬氨酸酶3(Cleaved caspase-3)和活化型半胱氨酸天冬氨酸酶9(Cleaved caspase-9)蛋白的表达。结果辣椒碱抑制LL/2细胞增殖,且具有浓度依赖性,作用48 h后其IC50值为125μmol·L^-1。实验组与对照组相比,细胞迁移能力显著降低、细胞核发生凝集和片段化。对照组与实验组的细胞克隆形成数量分别为35.00±1.73和15.00±1.20;这2组的细胞凋亡百分比分别为(9.23±0.21)%和(37.52±3.89)%;这2组的细胞质中细胞色素C分别为0.10±0.00和0.61±0.06;这2组的Cleaved-caspase-3分别为0.07±0.03和0.27±0.10;这2组的Cleaved-caspase-9的相对表达量分别为0.25±0.04和0.85±0.04,上述指标:组间比较差异均有统计学意义(均P<0.01)。结论辣椒碱可显著抑制小鼠Lewis肺癌细胞LL/2的增殖、迁移并能促进其凋亡,其机制可能是通过线粒体介导的途径诱导肿瘤细胞凋亡。Objective To study the effects of capsaicin on the proliferation,migration and apoptosis of mouse Lewis lung carcinoma cells LL/2 and explore its mechanism.Methods Mouse Lewis lung cancer cells LL/2 were divided into two groups:control group and experimental group.The experimental group was treated with different concentrations(25,50,100,150,200,250μmol·L^-1)of capsaicin with for 48 h;and the control cells were treated with the corresponding concentration of DMSO(Dimethyl sulfoxide).The cell viability and semi-inhibitory concentration(IC50)were detected by thiazolyl blue tetrazolium bromide(MTT)assay.The colony formation assay was used to detect changes in cell community after 10 days of treatment with 100μmol·L^-1 capsaicin.Annexin V-FITC/PI and Hoechst 33258 staining were used to detect apoptosis and nuclear morphological changes of cells treated with 125μmol·L^-1 capsaicin for 24 h.Western blotting was performed to detect the expression of Cytochrome C,Cleaved caspase-3 and Cleaved caspase-9 in the cytoplasm after treatment with 125μmol·L^-1 capsaicin for 24 h.Results Capsaicin inhibited LL/2 cell proliferation in a concentration-dependent manner,and its IC50 was 125μmol·L^-1 after 48 h treatment.Compared with the control group,the cell migration rate of the experimental group was significantly decreased,and the nucleus agglutinated and fragmented.The number of cell clones in control group and experimental group were 35.00±1.73 and 15.00±1.20;the percentage of apoptosis rate in the two groups were(9.23±0.21)%and(37.52±3.89)%;the expression levels of cytochrome C in the two groups were 0.10±0.00 and 0.61±0.06;the expression levels of Cleaved-caspase-3 in the two groups were 0.07±0.03 and 0.27±0.10;the expression levels of Cleaved-caspase-9 in the two groups were 0.25±0.04 and 0.85±0.04.Comparison between control group and experimental group,the differences of the factors were statistically significant(all P<0.01).Conclusion Capsaicin could significantly induce mouse Lewis lung cancer cell li

关 键 词：辣椒碱 LL/2细胞 增殖 凋亡 迁移 

分 类 号：R28[医药卫生—中药学]