海口不同农贸市场艾伯特埃希菌带染状况研究  

Study of contamination circumstance of Escherichia albertii in different farm markets in Haikou

作　　者：王艳燕[1] 闫国栋[2] 林春燕[1] 赵艺 张玲[2] 王红[2] WANG Yan-yan;YAN Guo-dong;LIN Chun-yan;ZHAO Yi;ZHANG Ling;WANG Hong(Haikou Municipal Center for Disease Control and Prevention,Haikou,Hainan 570102,China)

机构地区：[1]海南省海口市疾病预防控制中心,海南海口570102 [2]四川省自贡市疾病预防控制中心,四川自贡643000

出　　处：《中国卫生检验杂志》2019年第21期2655-2658,共4页Chinese Journal of Health Laboratory Technology

基　　金：自贡市重点科技计划项目(2013S06);四川省卫生计划和生育委员会科研课题(150259);传染病预防控制国家重点实验室开放课题(2016SKLID309)

摘　　要：目的了解海口市农贸市场家禽中艾伯特埃希菌的带染情况,为艾伯特埃希菌检测方法的有效性和可行性提供证据。方法采集海口市不同农贸市场市售鸡肠、鸭肠内容物经EC肉汤增菌后,PCR对其进行eae基因检测,阳性增菌液接种麦康凯琼脂,挑取eae阳性、不发酵乳糖菌落进行艾伯特埃希菌筛查后经三重PCR(clpX、lysP和mdh)及多位点序列分型(MLST)鉴定。结果本研究共采集了221份样品(鸡肠及其内容物133份,鸭肠及其内容物88份),从221份鸡肠、鸭肠内容物中检出11株疑似艾伯特埃希菌,经eae基因检测、生化鉴定、MLST聚类分析,结果表明该11株菌均为不发酵乳糖、木糖,无动力,eae基因和三重PCR为阳性,MLST聚类分析与艾伯特埃希菌标准菌株具有高度亲缘性,均鉴定为艾伯特埃希菌。结论海口市地区不同农贸市场家禽中艾伯特埃希菌存在不同程度的带染,且艾伯特埃希菌检测技术有效可行,具有推广应用价值。Objective To investigate the prevalence of Escherichia albertii in Haikou's farm market and provide basis to prove the method efficiency and feasibility of Escherichia albertii detection.Methods Enriched with EC broth,the retail duck and chicken intestines collected from different farm markets in Haikou were used for detecting eae gene by PCR.The positive EC broth in PCR detection of eae gene was inoculated in MacConkey.Those eae-positive lactose non-fermenting isolates were further classified as Escherichia albertii through triple PCR(clpXf lysP and mdh)and MLST(multilocus sequence typing).Results This study collected 221 samples including 133 chicken intestines and 88 duck intestines.Among 211 samples,there were 11 suspected Escherichia albertii detected.They were all non-fermenting lactose,xylose and defunct strains,whose results were eae-positive in triple PCR.After MLST cluster analysis,the 11 strains were highly related to the reference strain of Escherichia albertii and could be confirmed as Escherichia albertii.Conclusion This study indicated that Escherichia albertii existed in varying degrees in different farm markets in Haikou.Furthermore,the technology of Escherichia albertii detection was effective and feasible,which has applicable value.

关 键 词：艾伯特埃希菌 eae基因 三重PCR 多位点序列分型 

分 类 号：R446.5[医药卫生—诊断学]