玉米矮杆突变体的筛选与候选基因鉴定  

Screening of Dwarf Mutants and Identification of Candidate Genes in Maize

作　　者：王文秀 郑红艳 徐妙云[1] 邹俊杰 张兰[1] 王磊[1] WANG Wenxiu;ZHENG Hongyan;XU Miaoyun;ZOU Junjie;ZHANG Lan;WANG Lei(Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院生物技术研究所

出　　处：《中国农业科技导报》2019年第11期27-34,共8页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种培育重大专项(2016ZX08003002);中国农业科学院创新工程项目

摘　　要：挖掘矮杆基因对玉米耐密植品种选育具有重要意义。利用甲基磺酸乙酯(EMS)诱变郑58自交系获得一系列矮杆突变体,株高降低范围为10%~45%,穗位高降低范围为10%~65%,部分突变体的节间数目也发生了变化。对矮杆突变体细胞大小进行观察发现,突变体的细胞长度均缩短,缩短的范围为25%~75%,突变体株高的降低除了与细胞缩短有关外,还与细胞数目增加有关,为了进一步确定候选基因,对其中的5个突变体构建了F2分离群体,采取BSR高通量测序结合生物信息学分析,获得了多个玉米矮杆突变体的候选基因,为矮杆突变体基因的分离鉴定和开发应用提供了分析方法和新的材料。Exploring dwarf genes is very important for maize breeding to increase plant dense. In this study, a series of maize dwarf mutants were obtained by screening mutant library, which was constructed from inbred line Zheng 58 treated with ethyl methylate(EMS). Height of the dwarf mutants decreased by 10% ~ 45%, the spike height decreased by 10%~65%, and the number of internodes of some mutants was also changed. All of the dwarf mutants cell were shortened by 25% ~ 75%. Height of dwarf mutants was not only decided by cell shorting, also by cells number. To isolate dwarf candidate genes of mutants, five mutants were selected to construct the F2 segregation population. Combining BSR high-throughput sequencing and bioinformatics analysis, candidate genes of five mutants were obtained. These results provided an effective analytical method and new maize dwarf resource for dwarf gene isolation from mutants.

关 键 词：玉米 矮杆基因 甲基磺酸乙酯 突变体 BSR 

分 类 号：S513[农业科学—作物学] Q78[生物学—分子生物学]