Characterization of field-evolved resistance to cyantraniliprole in Bemisia tabaci MED from China  

作　　者：WANG Ran WANG Jin-da CHE Wu-nan SUN Yan LI Wen-xiang LUO Chen 

机构地区：[1]Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,P.R.China [2]State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agricultural and Forestry University,Fuzhou 350002,P.R.China [3]Department of Pesticide Sciences,Shenyang Agricultural University,Shenyang 110866,P.R.China [4]Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,P.R.China [5]College of Agriculture and Forestry Technology,Hebei North University,Zhangjiakou 075000,P.R.China

出　　处：《Journal of Integrative Agriculture》2019年第11期2571-2578,共8页农业科学学报（英文版）

基　　金：partly supported by research grants from the Scientific and Technological Innovation Capacity Construction Special Funds of the Beijing Academy of Agriculture and Forestry Sciences, China (KJCX20180705);the National Natural Science Foundation of China (31601635);the State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, China (SKL2018007);the Program on Application Basic Research Project of the Xinjiang Production and Construction Corps, China (2016AG004)

摘　　要：Cyantraniliprole is a novel anthranilic diamide insecticide with significant efficacy against Bemisia tabaci,an important pest insect worldwide.In this study,we conducted reversion and selection work and genetic analysis,and determined cross-resistance spectrum and synergism of cyantraniliprole resistance based on the reported population,SX population,of B.tabaci collected from Shanxi Province,China.Compared with a susceptible strain(MED-S),SX population,the field-evolved cyantraniliprole-resistant population exhibited 26.4-fold higher resistance to cyantraniliprole.In SX,a sharp decline of cyantraniliprole resistance was shown in the absence of selection.Another tested strain,SX-R,was established from SX population after successive selection with cyantraniliprole and recently developed 138.4-fold high resistance to cyantraniliprole.SX-R had no cross-resistance to abamectin,imidacloprid,thiamethoxam,sulfoxaflor,or bifenthrin.Genetic analysis illustrated that cyantraniliprole resistance in SX-R was autosomally inherited and incompletely dominant.Additionally,piperonyl butoxide(PBO)significantly inhibited cyantraniliprole resistance in the SX-R strain.In conclusion,the selection of SX with cyantraniliprole led to high resistance to cyantraniliprole which is incompletely dominant and no cross-resistance to several common types of insecticides.Enhanced oxidative metabolism is possibly involved in the resistance of SX-R,yet target-site resistance could not be excluded.Cyantraniliprole is a novel anthranilic diamide insecticide with significant efficacy against Bemisia tabaci, an important pest insect worldwide. In this study, we conducted reversion and selection work and genetic analysis, and determined cross-resistance spectrum and synergism of cyantraniliprole resistance based on the reported population, SX population, of B. tabaci collected from Shanxi Province, China. Compared with a susceptible strain(MED-S), SX population, the field-evolved cyantraniliprole-resistant population exhibited 26.4-fold higher resistance to cyantraniliprole. In SX, a sharp decline of cyantraniliprole resistance was shown in the absence of selection. Another tested strain, SX-R, was established from SX population after successive selection with cyantraniliprole and recently developed 138.4-fold high resistance to cyantraniliprole. SX-R had no cross-resistance to abamectin, imidacloprid, thiamethoxam, sulfoxaflor, or bifenthrin. Genetic analysis illustrated that cyantraniliprole resistance in SX-R was autosomally inherited and incompletely dominant. Additionally, piperonyl butoxide(PBO) significantly inhibited cyantraniliprole resistance in the SX-R strain. In conclusion, the selection of SX with cyantraniliprole led to high resistance to cyantraniliprole which is incompletely dominant and no cross-resistance to several common types of insecticides. Enhanced oxidative metabolism is possibly involved in the resistance of SX-R, yet target-site resistance could not be excluded.

关 键 词：BEMISIA TABACI cyantraniliprole CROSS-RESISTANCE INHERITANCE synergism 

分 类 号：F42[经济管理—产业经济]