Sysmex XN-1000自动血细胞分析仪复检规则的建立与评估  

Establishment and evaluation of review criteria for Sysmex XN-1000 automated blood cell analyzer

作　　者：薛桂阳 曹岩[1] 刘明开[1] Xue Guiyang;Cao Yan;Liu Mingkai(Department of Clinical Laboratory,Xinhua Hospital Affiliated to Dalian University,Dalian 116021,Liaoning,China)

机构地区：[1]大连大学附属新华医院检验科

出　　处：《实用检验医师杂志》2019年第3期154-157,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的建立适合本实验室的Sysmex XN-1000血液分析仪复检规则并对其进行评价。方法采用日本Sysmex XN-1000分析仪,随机检测大连大学附属新华医院住院患者的1241份血液标本,按照41条复检规则和涂片镜检阳性标准进行评估,计算真阳性率、假阳性率、真阴性率和假阴性率,建立适合本实验室的血细胞分析复检规则。结果本套复检规则的真阳性率为17.0%(211/1241)、假阳性率为11.3%(140/1241)、真阴性率为68.9%(855/1241)、假阴性率为2.8%(35/1241)、复检率为27.7%(344/1241),无血液病漏检的情况。结论根据本科室情况自定义Sysmex XN-1000的复检规则能够有效筛选异常标本,提高工作效率,还仍需在应用中进行完善。Objective To establish and evaluate the suitable review criteria of Sysmex XN-1000 hematologic analyzer for our laboratory.Methods A Japanese Sysmex XN-1000 analyzer was used for random detection of 1241 patients'blood samples collected from Xinhua Hospital Affiliated to Dalian University,and according to the 41 international rules for retest and the positive criteria of microscopic examination of slide smear,the evaluation was carried out and the true and false positive rates,true and false negative rates were calculated to establish the retest rules of blood cells analysis suitable to our laboratory.Results According to our self-made"rules 18",the true positive rate was 17.0%(211/1241),the false positive rate was 11.3%(140/1241),the true negative rate was 68.9%(855/1241),the false negative rate was 2.8%(35/1241),the smear review rate was 27.7%(344/1241),and in regard to blood diseases,no un-detection situation was present.Conclusion According to the situation of our laboratory,the self-made review rules for Sysmex XN-1000 hematologic analyzer can effectively screen out the abnormal specimens and elevate working efficiency,however,it is still necessary to improve the rules through practices.

关 键 词：细胞计数 血细胞复检规则 血细胞形态 评价研究 

分 类 号：R44[医药卫生—诊断学]