免疫比浊法与阳离子交换层析法检测糖化血红蛋白结果的对比分析  

Comparative analyses of immunoturbidimetric assay and cation exchange chromatography for detection of glycosylated hemoglobin

作　　者：唐漫乔 Tang Manqiao(Department of Clinical Laboratory,Lianxia Central Hospital of Chenghai District,Shantou 515834,Guangdong,China)

机构地区：[1]汕头市澄海区莲下中心医院检验科

出　　处：《实用检验医师杂志》2019年第3期169-172,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的对比分析免疫比浊法(TIIA)和阳离子交换层析法(IEC)对全血糖化血红蛋白(HbAlc)的检测价值。方法选择2018年1月—2019年2月汕头市澄海区莲下中心医院收治的168例2型糖尿病(T2DM)患者作为研究对象,分别采用TIIA法和IEC法检测HbAlc水平。设Y为IEC法所测HbAlc浓度水平,X为TIIA法所测HbAlc浓度水平,绘制回归方程曲线,比较两种检测方法的精密度试验、线性相关分析和回收试验结果。结果HbA1c精密度检测和回收试验中,IEC法的平均回收率(96.99%)、批内变异系数(CV批内,3.43%)和批间变异系数(CV批间,4.19%)均明显优于TIIA法(分别为92.86%、9.67%、10.67%,均P<0.05);相关性分析显示,IEC法(Y)和TIIA法(X)的相关性分析回归方程为Y=0.97X+1.23,R^2=0.991。结论从方法学角度分析,IEC法检测全血HbAlc的精密度优于TIIA法,且与TIIA法的相关性较好,同时IEC法可动态观察T2DM疗效和监测病情,具有准确率高、快速简便的特点,适合临床推广使用。Objective To compare and analyze the detection values of immunoturbidimetric(TIIA)assay and cation exchange chromatography(IEC)for the detection of total glycosylated hemoglobin(HbA1c).Methods A total of 168 patients with typeⅡdiabetes(T2DM)admitted to Lianxia Central Hospital in Chenghai District,Shantou City from January 2018 to February 2019 were selected as the research subjects,and their HbA1c levels were detected using TIIA method and IEC method respectively.The Y and X were assigned to be the HbAlc concentration levels measured by IEC and TIIA methods respectively,the regression equation curve was drawn,and the results of precision test,linear correlation analysis and recovery test of the two methods were compared.Results In the tests of HbA1c precision degree and recovery rate,the average recovery rate(96.99%),variable coefficient(CV)within batch(3.43%)and the CV among batches(4.19%)of the IEC method were significantly superior to those in the TIIA method(respectively 92.86%,9.67%and 10.67%,all P<0.05).The correlation analysis indicated that the regression equation of IEC method(Y)and TIIA method(X)was as follows:Y=0.97X+1.23,R^2=0.991.Conclusion From the methodological analysis point of view,using the IEC method to detect the precision degree of HbAlc in the whole blood is superior to that of TIIA method,and the correlation between IEC and TIIA method is relatively good.At the same time,the IEC method can dynamically observe the therapeutic effect and monitor the disease conditions of the patients with T2DM,thus IEC possesses the high accuracy rate,fast and simple characteristics,which is suitable for its wide application in clinics.

关 键 词：免疫比浊法 阳离子交换层析法 糖化血红蛋白 2型糖尿病 

分 类 号：R58[医药卫生—内分泌]