9%嘧肟·氰氟草乳油高效液相色谱分析  

Determination of Pyribenzoxim·Cyhalofop-butyl 9% EC by HPLC

作　　者：李向阳[1] 倪立霞 姜士聚[1] 张耀中[1] LI Xiang-yang;NI Li-xia;JIANG Shi-ju;ZHANG Yao-zhong(Institute for the Control of Agrochemicals of Shandong Province,Jif nan 250100,China;Shandong Kesaijinong Biotechnology Co.,Ltd.,Ji'nan 250101,China)

机构地区：[1]山东省农药检定所,济南250100 [2]山东科赛基农生物科技有限公司,济南250101

出　　处：《农药》2019年第11期816-818,823,共4页Agrochemicals

摘　　要：[目的]建立快速灵敏检测嘧啶肟草醚、氰氟草酯的高效液相色谱定量分析方法。[方法]采用以Li Chrosher RP18为填充物的不锈钢柱和紫外检测器,用乙腈溶解试样,以乙腈-水-磷酸为流动相,对试样中嘧啶肟草醚进行分离和定量分析。采用以Chiralcel OJ-H为填充物的不锈钢柱和紫外检测器,以正己烷-异丙醇为流动相并用流动相溶解试样,对试样中氰氟草酯进行分离和定量分析。[结果]嘧啶肟草醚、氰氟草酯分析方法的线性相关系数分别为0.999、1.000,标准偏差分别为0.0063、0.0210,变异系数为0.23%、0.32%,平均回收率为99.74%、99.50%。[结论]该分析方法使得嘧啶肟草醚、氰氟草酯得到较好分离,操作简单、快速,定量分析结果准确、稳定,可用于嘧啶肟草醚、氰氟草酯的精密定量分析。[Aims]A method for the quantitative determination of pyribenzoxim·cyhalofop-butyl by HPLC was developed.[Methods]It was established by using Li Chrosher RP18(5μm)and UV detector for isolating and determinating pyribenzoxim.The sample was dissolved in acetonitrile,and was determinated by using acetonitrile,water and phosphoric acid as the mobile phase.It was established by using chiralcel OJ-H(5μm)and UV detector for isolating and determinating cyhalofop-butyl,and dissolving the sample with mobile phase and using N-hexane and isopropyl alcohol as the mobile phase.[Results]The linear correlation coefficient of pyribenzoxim and cyhalofop-butyl were 0.999 and 1.000,the standard deviation were 0.0063 and 0.0210,the variation coefficient were 0.23 and 0.32%,the average recovery were99.74 and 99.50%.[Conclusions]This method is simple,fast,high precision and accuracy,which can meet the requirements for quantitative determination of pyribenzoxim and cyhalofop-butyl.

关 键 词：嘧啶肟草醚 氰氟草酯 高效液相色谱 分析方法 

分 类 号：TQ450.7[化学工程—农药化工]