云南6个产地滇丹参植株地上部分3种水溶性活性成分含量HPLC-PDA测定  

Determination of 3 Hydrophillic Components in the overground part of Salvia yunnanensis C. H. Wright Originate From Six Regions inYunnan by HPLC-PDA

作　　者：朱培芳 孙海林 唐修文 饶高雄 ZHU Pei-fang;SUN Hai-lin;TANG Xiu-wen;RAO Gao-xiong(College of Pharmaceutical Science,Yunnan University of Chinese Medicine,Kunming 650500,China;Yunnan Daphne Pharmaceutical Co.Ltd,Wenshan 663000,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]云南特安呐制药股份有限公司,云南文山663000

出　　处：《时珍国医国药》2019年第9期2263-2267,共5页Lishizhen Medicine and Materia Medica Research

基　　金：云南省科技创新强省计划(社会发展)项目(2013AE003)

摘　　要：目的建立高效液相色谱法(PDA检测器)同时测定云南6个产地滇丹参植株地上部分3种水溶性活性成分含量,为滇丹参的合理开发和全株植物的利用提供依据。方法戴安U3000系列高效液相色谱仪(PDA检测器),ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.2%磷酸缓冲溶液梯度洗脱,流速1.2 mL/min,检测波长280nm、286nm和330nm,柱温箱25℃。结果三种水溶性成分均呈较好的回归方程和相关系数,相关系数丹参素为0.9979,丹酚酸B和迷迭香酸为0.9999。其中丹参素进样范围0.472~1.770μg,丹酚酸B进样范围0.648~2.430μg,迷迭香酸进样范围2.960~11.100μg。丹参素平均回收率为98.703%,丹酚酸B平均回收率为99.475%,迷迭香酸平均回收率为98.935%。结论云南6个产地滇丹参植株地上部分水溶性成分含量有很大差异,嵩明产药材植株地上部分水溶性成分含量最高,其中迷迭香酸含量最高。新平产药材植株地上部分中丹酚酸B含量也相对较高,6个产地中药材植株地上部分中都含这3种水溶性成分。建立了另一色谱方法,可同时测定滇丹参中11种化学成分,包括脂溶性成分3和水溶性成分8种。方法简单准确高效稳定性好,可为滇丹参地方资源及药材全株的合理开发利用提供依据。Objective To establish a method for determination of 3 hydrophillic components in the overground part of Salvia yunnanensis C.H.Wright originate from six regions in Yunnan by HPLC-PDA.It provides the basis for rational exploitation and utilization of the resources of Yunnan.Methods The Ultimate U3000 series high performance liquid chromatograph(PDA detector),ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm,5μm),using acetonitrile-water(containing 0.2%phosphoric acid)in the gradient mode,the flow rate was 1.2mL/min,the wavelength of UV detector was set at 280nm,286nm and 330nm,the column temperature was set at 25℃.Results The relationship between the concentration and the peak areas of the three compounds was all linear.The correlation coefficient reaches 0.9979~0.9999.The average recovery rate was 98.703%~99.475%.Conclusion The method is accurate,high precision,good stability,can provide Salvia yunnanensis C.H.Wright content determination and the development and utilization of local resources in medicine to provide important reference and scientific basis.

关 键 词：滇丹参 水溶性成分 HPLC-PDA 

分 类 号：R284.2[医药卫生—中药学]