水稻籽粒γ-氨基丁酸含量的QTL定位分析  

QTL Mapping for the Genetic Components Determining the Rice Grain γ-Aminobutyric Acid Content

作　　者：田玲 王康恺 王迎超 杨治伟[1,2] 马嘉欣 赵娜 田普江 田蕾[1,2] 张银霞 杨淑琴 李培富[1,2] TIAN Ling;WANG Kang-kai;WANG Ying-chao;YANG Zhi-wei;MA Jia-xin;ZHAO Na;TIAN Pu-jiang;TIAN Lei;ZHANG Yin-xia;YANG Shu-qin;LI Pei-fu(Key Laboratory of Modern Molecular Breeding of Advantageous Crops of Ningxia,Yinchuan 750021;College of Agronomy,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏优势特色作物现代分子育种重点实验室,银川750021 [2]宁夏大学农学院,银川750021

出　　处：《植物遗传资源学报》2019年第6期1517-1522,共6页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31360324,31760374,31401361);宁夏农业育种专项课题(2018NYYZ0302)~~

摘　　要：本研究以低含量γ-氨基丁酸的宁农黑粳为母本,高含量γ-氨基丁酸的高粱稻-1为父本,构建F2群体,获得了216个F2单株。利用130个SSR标记构建了一张F2群体的SSR标记连锁图谱,覆盖基因组长度为2406.9 cM,连锁群长度在129.5~360.7 cM之间,标记间的平均距离为18.5 cM,并进一步开展控制水稻γ-氨基丁酸含量的QTL定位研究。结果表明:共检测到7个QTL位点,分别位于第8号和第9号染色体上,其中qGABA8-2、qGABA8-3、qGABA9-1的贡献率依次为10%、11%和9%。对3个贡献率大的QTL位点进行复合区间作图,当LRS为25.6时,在RM342~RM515处可能存在较为可靠的QTL,初步将qGABA8定位在标记RM342与RM515之间的326 kb区间内。利用InDel标记对目标区间加密,将该区间进一步缩小到183 kb区间内,位于标记RM342和G121之间。本研究结果可进一步通过构建次级群体对该基因进行精细定位及图位克隆,同时,研究中筛选出的SSR标记和设计的InDel标记可快速筛选水稻育种材料中富γ-氨基丁酸的基因型,加快育种进程。The rice cultivars Ningnong black carp and high indica rice exhibited low and high content on γ-aminobutyric acid,respectively.Both genotypes were deployed for generating a F2 population that comprises of 216 individual plants.By taking use of 130 polymorphic SSR markers,a linkage map was produced with an length of 2406.9 cM.Each linkage group expands from 129.5 cM to 360.7 cM,and a mean distance between markers was 18.5 cM.The QTL mapping positioning study showed that seven QTL loci controlling the γ-aminobutyric acid content were found on chromosomes 8 and 9,respectively.The contribution rate of qGABA8-2,qGABA8-3,qGABA9-1 was 10%,11% and 9%,respectively.The composite interval mapping was performed on three QTL sites with large contribution rate.When LRS was 25.6,a QTL qGABA8 was initially found at RM342-RM515 in an interval of 326 kb.The confidence interval was further delimited to 180 kb by using the InDel marker G121.Therefore,the accumulated results of this study might be useful in further gene isolation and marker-assisted screening for the genotypes with the enriched amino butyric acid.

关 键 词：水稻 Γ-氨基丁酸 SSR标记 QTL定位 

分 类 号：S51[农业科学—作物学]